bs-20596R-A647 [Conjugated Primary Antibody]
Cyclin D1, Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cyclin D1,

Immunogen Range: 101-200/295


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 595

Swiss Prot: P24385

Source: KLH conjugated synthetic peptide derived from human Cyclin D1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008].

Conjugation: ALEXA FLUOR® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 32


Cross Reactive Species: Human
Mouse
Rat
Bovine

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jiang L et al. Astrocytes induce proliferation of oligodendrocyte progenitor cells via connexin 47-mediated activation of Chi3l1 expression. Eur Rev Med Pharmacol Sci. 2019 Apr;23(7):3012-3020. Read more>>
  • "Zou P et al. Mechanisms of Stress-Induced Spermatogenesis Impairment in Male Rats Following Unpredictable Chronic Mild Stress (uCMS). Int. J. Mol. Sci. 2019, 20, 4470."Read more>>
  • "Zou P et al. Mechanisms of Stress-Induced Spermatogenesis Impairment in Male Rats Following Unpredictable Chronic Mild Stress (uCMS). Int. J. Mol. Sci. 2019, 20, 4470."Read more>>
  • Jiancong Lu. et al. Circ_0020123 Increases ZFX Expression to Facilitate Non-Small Cell Lung Cancer Progression by Sponging miR-142-3p. Cancer Manag Res. 2021; 13: 1687C1698Read more>>
  • Zhang Wei. et al. C1QTNF6 regulates cell proliferation and apoptosis of NSCLC in vitro and in vivo. Bioscience Rep. 2021 Jan;41(1):BSR20201541Read more>>
  • Yunqi Hua. et al. Circular RNA Circ_0006282 Promotes Cell Proliferation and Metastasis in Gastric Cancer by Regulating MicroRNA-144-5p/Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Axis. Cancer Manag Res. 2021; 13: 815C827Read more>>
  • Juan Tan. et al. Circ_0124644 Serves as a ceRNA for miR-590-3p to Promote Hypoxia-Induced Cardiomyocytes Injury via Regulating SOX4. Front Genet. 2021; 12: 667724Read more>>
  • Jing Wu. et al. The role of ER stress and ATP/AMPK in oxidative stress meditated hepatotoxicity induced by citrinin. ECOTOX ENVIRON SAFE. 2022 Jun;237:113531Read more>>
  • Cong Lan. et al. Inhibition of DYRK1A, via histone modification, promotes cardiomyocyte cell cycle activation and cardiac repair after myocardial infarction. EBIOMEDICINE. 2022 Aug;82:104139Read more>>
  • Huang Weizhen. et al. LncRNA SNHG11 enhances bevacizumab resistance in colorectal cancer by mediating miR-1207-5p/ABCC1 axis. ANTI-CANCER DRUG. 2022 Jul;33(6):575-586Read more>>
  • Xiaoyv Yue. et al. circITGB1 Regulates Adipocyte Proliferation and Differentiation via the miR-23a/ARRB1 Pathway. INT J MOL SCI. 2023 Jan;24(3):1976Read more>>
  • Jing Liu. et al. Exosomes Promote Atherosclerosis Progression by Regulating Circ_100696/miR-503-5p/PAPPA Axis-Mediated Vascular Smooth Muscle Cells Proliferation and Migration. INT HEART J. 2023 Sep;64(5):918-927Read more>>
VALIDATION IMAGES