bs-20613R [Primary Antibody]
Caspase-8 subunit p10 Polyclonal Antibody
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DATASHEET

Host: Rabbit

Target Protein: Caspase-8 subunit p10

Immunogen Range: 351-420/480


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 18669

Swiss Prot: O89110

Source: KLH conjugated synthetic peptide derived from mouse Caspase 8

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Subunit : Interacts with PSMB5.

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(ICC)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu F et al. Anticancer targets and mechanisms of calycosin to treat nasopharyngeal carcinoma. Biofactors. 2020 Jul;46(4):675-684.Read more>>
  • Furui Han. et al. In vivo and in vitro study on hepatotoxicity of Tris-(2, 3-dibromopropyl) isocyanurate exposure via mitochondrial and death receptor pathway. ECOTOX ENVIRON SAFE. 2022 Nov;246:114186Read more>>
VALIDATION IMAGES

Mouse Thymus lysates probed with Caspase 8 Polyclonal Antibody, Unconjugated (bs-20613R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Raw264.7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase-8 subunit p10) polyclonal Antibody, Unconjugated (bs-20613R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.