DATASHEET
Host:
Rabbit
Target Protein:
Caspase-8 subunit p10
Immunogen Range:
351-420/480
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
18669
Swiss Prot:
O89110
Source:
KLH conjugated synthetic peptide derived from mouse Caspase 8
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C for 12 months.
Background:
Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Subunit : Interacts with PSMB5.
PRODUCT SPECIFIC PUBLICATIONS
- Liu F et al. Anticancer targets and mechanisms of calycosin to treat nasopharyngeal carcinoma. Biofactors. 2020 Jul;46(4):675-684.Read more>>
- Furui Han. et al. In vivo and in vitro study on hepatotoxicity of Tris-(2, 3-dibromopropyl) isocyanurate exposure via mitochondrial and death receptor pathway. ECOTOX ENVIRON SAFE. 2022 Nov;246:114186Read more>>
VALIDATION IMAGES
Mouse Thymus lysates probed with Caspase 8 Polyclonal Antibody, Unconjugated (bs-20613R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Raw264.7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase-8 subunit p10) polyclonal Antibody, Unconjugated (bs-20613R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.