bs-20627R [Primary Antibody]
Androgen receptor Polyclonal Antibody
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: Androgen receptor

Immunogen Range: 701-800/919


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 367

Swiss Prot: P10275

Source: KLH conjugated synthetic peptide derived from human Androgen receptor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1ug/ul

Applications: IHC-P(1:200-400)
IHC-F(1:100-500)
IF(ICC)(1:50-200)
IF()

Predicted Molecular Weight: 43/101 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shen H et al. Effects of elevated ambient temperature and local testicular heating on the expressions of heat shock protein 70 and androgen receptor in boar testes. Acta Histochem. 2019 Feb 2. pii: S0065-1281(18)30339-8. Read more>>
  • Duan H et al. Expression of estrogen receptor, androgen receptor, and progesterone nuclear receptor in sheep uterus during the estrous cycle. Reprod Domest Anim. 2019 Jun 12. Read more>>
  • Xiao L et al. Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression. J Cell Physiol. 2019 Jan 22. Read more>>
  • Song L et al. DHT and E2 synthesis-related proteins and receptors expression in male yak skin during different hair follicle stages. Gen Comp Endocrinol. 2019 Aug 12:113245. Read more>>
  • Jie Su. et al. Study on the changes of LHR, FSHR and AR with the development of testis cells in Hu sheep. ANIM REPROD SCI. 2023 Sep;256:107306Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Androgen receptor Polyclonal Antibody, Unconjugated (bs-20627R) at 1:400 overnight at 4°C, DAB staining.