Host: Rabbit
Target Protein: CD20
Immunogen Range: 101-200/297
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 931
Swiss Prot: P11836
Source: KLH conjugated synthetic peptide derived from human CD20
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
Predicted Molecular Weight: 33
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species: Rat
For research use only. Not intended for diagnostic or therapeutic use.
Lane 1, spleen lysates, Lane 2, Lymph node lysate probed with CD20 Polyclonal Antibody (bs-20638R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C
Mouse spleen cells probed with CD20 Polyclonal Antibody, unconjugated (bs-20638R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
Lane 1: Mouse Large intestine lysates; Lane 2: Mouse Bone lysates probed with CD20 Polyclonal Antibody, Unconjugated (bs-20638R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.