bs-2066R-A647 [Conjugated Primary Antibody]
GSK-3 Beta(Ser9) Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]

Host: Rabbit

Target Protein: GSK-3 Beta Ser9

Modification Site: Ser9

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2932

Swiss Prot: P49841

Source: KLH conjugated synthetic phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Ser9

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at 4°C for 12 months.

Background: The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Conjugation: ALEXA FLUOR® 647

Excitation/ Emission: 650nm/665nm

Size: 100ul

Concentration: 1ug/ul

Applications: FCM(1:20-100)

Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

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  • Tong Xu. et al. Lithium chloride represses abdominal aortic aneurysm via regulating GSK3/SIRT1/NF-B signaling pathway. Free Radical Bio Med. 2021 Apr;166:1Read more>>
  • Peiqi Zhu. et al. Panax notoginseng saponins promote endothelial progenitor cell angiogenesis via the Wnt/-catenin pathway. Bmc Complem Altern M. 2021 Dec;21(1):1-11Read more>>
  • Qingxin Fan. et al. Ginsenoside Rb1 Facilitates Browning by Repressing Wnt/-Catenin Signaling in 3T3-L1 Adipocytes. Med Sci Monitor. 2021; 27: e928619-1Ce928619-1Read more>>

Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (phospho-GSK-3 Beta (Ser9)) polyclonal Antibody, Unconjugated (bs-2066R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXCL2 Antibody(bs-R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).