bs-20678R [Primary Antibody]
CD146 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD146

Immunogen Range: 501-600/646


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4162

Swiss Prot: P43121

Source: KLH conjugated synthetic peptide derived from human CD146

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

MCAM (MUC18 antigen, CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MCAM (Melanoma adhesion molecule) has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MCAM. The close structural relationship with N-CAM and related molecules suggests that MCAM may be also a developmentally regulated cell adhesion.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 71


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse spleen lysates probed with CD146 Polyclonal Antibody, Unconjugated (bs-20678R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse trachea lysates; Lane 2: Mouse Liver lysates probed with CD146 Polyclonal Antibody, Unconjugated (bs-20678R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C. Lower band is isoform 2;