bs-20687R [Primary Antibody]
ROR alpha Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ROR alpha

Immunogen Range: 321-420/523


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6095

Swiss Prot: P35398

Source: KLH conjugated synthetic peptide derived from human ROR alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 59


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

U251 cell lysates probed with ROR alpha Polyclonal Antibody, Unconjugated (bs-20687R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Mouse kidney lysates probed with ROR alpha Polyclonal Antibody, Unconjugated (bs-20687R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Mouse Liver lysates probed with ROR alpha Polyclonal Antibody, Unconjugated (bs-20687R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Heart tissue lysates; Lane 5: Mouse Liver tissue lysates; Lane 6: Rat Cerebellum tissue lysates; Lane 7: Rat Heart tissue lysates; Lane 8: Human HeLa cell lysates probed with ROR alpha Polyclonal Antibody, Unconjugated (bs-20687R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.