bs-2072R [Primary Antibody]
NOS-2/iNOS Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: iNOS

Immunogen Range: 14-100/1144


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 18126

Swiss Prot: P29477

Source: KLH conjugated synthetic peptide derived from mouse NOS-2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 130


Cross Reactive Species: Human
Mouse
Rat
Chicken

Predicted Cross Reactive Species: Dog
Pig
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sun, Jing, et al. "Alpha-lipoic acid attenuates trinitrobenzene sulfonic acid-induced ulcerative colitis in mice." International journal of clinical and experimental medicine 8.1 (2015): 358-367.Read more>>
  • Karadeniz, Tugba, et al. "Experimental comparison of protective characteristics of enalapril and trimetazidine in diabetic nephropathy." Renal failure 36.8 (2014): 1283-1290.Read more>>
  • Bai, Lijuan, et al. "Somatostatin prevents lipopolysaccharide‑induced neurodegeneration in the rat substantia nigra by inhibiting the activation of microglia." Molecular Medicine Reports.Read more>>
  • Wang, Haifeng, et al. "Seawater immersion aggravates sciatic nerve injury in rats." Experimental and therapeutic medicine 9.4 (2015): 1153-1160.Read more>>
  • Zhao, Meng, et al. "The Roles of Reactive Oxygen Species and Nitric Oxide in Perfluorooctanoic Acid-Induced Developmental Cardiotoxicity and l-Carnitine Mediated Protection." International Journal of Molecular Sciences 18.6 (2017): 1229.Read more>>
  • Sun, Guodong, et al. "Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats." Experimental Animals: 16-0102.Read more>>
  • Liu, Qiang, et al. "Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis." Biomedicine & Pharmacotherapy(2017).Read more>>
  • Huang et al. RANKL-induced M1 macrophages are involved in bone formation. (2017) Bone.Res. 5:17019Read more>>
  • Zhao et al. Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-κB Signaling Pathway. (2016) Evid.Based.Complement.Alternat.Med. 2016:8219287Read more>>
  • Xiong et al. Salvianolic Acid B Restored Impaired Barrier Function via Downregulation of MLCK by microRNA-1 in Rat Colitis Model. (2016) Front.Pharmacol. 7:134Read more>>
  • Li et al. Autologous transplantation of adipose-derived mesenchymal stem cells attenuates cerebral ischemia and reperfusion injury through suppressing apoptosis and inducible nitric oxide synthase. (2012) Int.J.Mol.Med. 29:848-54Read more>>
  • Zhao et al. Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats. (2016) J.Histochem.Cytochem. 64:125-37Read more>>
  • Shao,et al.Arsenic and/or copper caused inflammatory response via activation of inducible nitric oxide synthase pathway and triggered heat shock protein responses in testis tissues of chicken.(2018) Environmental Science and Pollution Research. 25:7719-7729.Read more>>
  • Yang,et al.Aptamer-Based Dual-Functional Probe for Rapid and Specific Counting and Imaging of MCF-7 Cells.(2018) Analytical Chemistry. 90:2355-2361.Read more>>
  • Vairappan B et al. Resveratrol Restores Neuronal Tight Junction Proteins Through Correction of Ammonia and Inflammation in CCl4-Induced Cirrhotic Mice. Molecular Neurobiology. Oct 30.Read more>>
  • EMŐKE HORVÁTH et al. FISH-OIL PRECONDITIONING UP-REGULATES EXPRESSION OF SPLENIC ARG1 POSITIVE M2 TYPE MACROPHAGES AND THE ARG1/INOS2 RATIO AFTER EXPERIMENTAL INDUCED TRANSIENT CEREBRAL ISCHEMIA. FARMACIA, 2019, Vol. 67, 5.Read more>>
  • Saghir SAM et al. Thymoquinone-PLGA-PVA Nanoparticles Ameliorate Bleomycin-Induced Pulmonary Fibrosis in Rats via Regulation of Inflammatory Cytokines and iNOS Signaling. Animals (Basel). 2019 Nov 11;9(11). pii: E951.Read more>>
  • Yang X et al. The Activation of Heat-Shock Protein After Copper (II) and/or Arsenic (III)-Induced Imbalance of Homeostasis, Inflammatory Response in Chicken Rectum. Biol Trace Elem Res. 2019 Aug 31.Read more>>
  • Wu TH et al. The Combination of Astragalus membranaceus and Angelica sinensis Inhibits Lung Cancer and Cachexia through Its Immunomodulatory Function. J Oncol. 2019 Nov 3;2019:9206951. Read more>>
  • Wedn AM et al. Time and sex dependency of hemodynamic, renal, and survivability effects of endotoxemia in rats. Saudi Pharm J. 2020 Jan;28(1):127-135.Read more>>
  • ?zel F et al. Protective effect of alpha lipoic acid on 4-vinylcyclohexene diepoxide induced primary ovarian failure in female rats. Taiwan J Obstet Gynecol. 2020 Mar;59(2):293-300. Read more>>
  • Tong?L et al. ACT001 reduces the expression of PD-L1 by inhibiting the phosphorylation of STAT3 in glioblastoma. Theranostics. 2020 May 1;10(13):5943-5956.Read more>>
  • Toru Nishikawa et al. Therapeutic potential for insulin on type?1 diabetes\associated periodontitis: Analysis of experimental periodontitis in streptozotocin\induced diabetic rats. J Diabetes Investig. 2020 Nov;11(6):1482-1489.Read more>>
  • Yang Fan. et al. Hirudo Lyophilized Powder Ameliorates Renal Injury in Diabetic Rats by Suppressing Oxidative Stress and Inflammation. Evid-Based Compl Alt. 2021;2021:6657673Read more>>
  • Shih-Yuan Fang. et al. Distinct phenotypic expression levels of macrophages in neonatal lungs. Exp Ther Med. 2021 Apr;21(4):1-8Read more>>
  • Acikgoz Burcu. et al. The effects of prenatal and postnatal exposure to electromagnetic field on rat ovarian tissue:. Toxicol Ind Health. 2020;36(12):1010-1018Read more>>
  • Mahmoud E. Youssef. et al. 7-nAChRs-mediated therapeutic angiogenesis accounts for the advantageous effect of low nicotine doses against myocardial infarction in rats. Eur J Pharmacol. 2021 Mar;:173996Read more>>
  • Kohei Kamada. et al. Attenuation of Knee Osteoarthritis Progression in Mice through Polarization of M2 Macrophages by Intra-Articular Transplantation of Non-Cultured Human Adipose-Derived Regenerative Cells. J Clin Med. 2021 Jan;10(19):4309Read more>>
  • Ali Tu?rul Akin. et al. Therapeutic effects of thymoquinone in doxorubicin-induced hepatotoxicity via oxidative stress, inflammation and apoptosis. 2021 Sep 08Read more>>
  • Yang Sun. et al. Dual Biosignal-Functional Injectable Microspheres for Remodeling Osteogenic Microenvironment. SMALL. 2022 Apr 14Read more>>
  • Juin-Hong Cherng. et al. Hemostasis and Anti-Inflammatory Abilities of AuNPs-Coated Chitosan Dressing for Burn Wounds. J PERS MED. 2022 Jul;12(7):1089Read more>>
  • Derya Kaya. et al. Allopurinol attenuates repeated traumatic brain injury in old rats: A preliminary report. EXP NEUROL. 2022 Nov;357:114196Read more>>
  • Necla Deger. et al. Thymoquinone regulates nitric oxide synthase enzymes and receptor-interacting serine-threonine kinases in isoproterenol-induced myocardial infarcted rats. CHEM-BIOL INTERACT. 2022 Aug;:11009Read more>>
  • Wenbo Ge. et al. Melatonin protects sheep endometrial epithelial cells against lipopolysaccharide-induced inflammation in vitro. REPROD DOMEST ANIM. 2022 AugRead more>>
  • Yang Liu. et al. Platycodon grandiflorus polysaccharides deeply participate in the anti-chronic bronchitis effects of platycodon grandiflorus decoction, a representative of the lung and intestine are related. FRONT PHARMACOL. 2022 Sep 7;13:927384Read more>>
  • Hager S. Zoghroban. et al. The impact of L-citrulline on murine intestinal cell integrity, immune response, and arginine metabolism in the face of Giardia lamblia infection. ACTA TROP. 2023 Jan;237:106748Read more>>
  • Xiao Wang. et al. Salvia miltiorrhiza polysaccharides alleviate florfenicol-induced inflammation and oxidative stress in chick livers by regulating phagosome signaling pathway. ECOTOX ENVIRON SAFE. 2023 Jan;249:114428Read more>>
  • Dan-dan Wu. et al. STING mediates SU5416/hypoxia-induced pulmonary arterial hypertension in rats by regulating macrophage NLRP3 inflammasome activation. IMMUNOBIOLOGY. 2023 Mar;228:152345Read more>>
  • Duchenhui Li. et al. The immunomodulatory effect of IL-4 accelerates bone substitute material-mediated osteogenesis in aged rats via NLRP3 inflammasome inhibition. FRONT IMMUNOL. 2023; 14: 1121549Read more>>
  • Changjun Chen. et al. Engineered Exosome-Functionalized Extracellular Matrix-Mimicking Hydrogel for Promoting Bone Repair in Glucocorticoid-Induced Osteonecrosis of the Femoral Head. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Yanhong Li. et al. Allogeneic Adipose-Derived Mesenchymal Stem Cell Transplantation Alleviates Atherosclerotic Plaque by Inhibiting Ox-LDL Uptake, Inflammatory Reaction and Endothelial Damage in Rabbits. CELLS-BASEL. 2023 Jan;12(15):1936Read more>>
  • Jung-Eun Kim. et al. Efficacy of FRO on Acne Vulgaris Pathogenesis. PHARMACEUTICS. 2023 Jul;15(7):1885Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin-embedded : rat brain labeled with Rabbit Anti-NOS-2/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-NOS-2/iNOS Polyclonal Antibody, Unconjugated(bs-2072R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C and DAPI


Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NOS-2/iNOS Polyclonal Antibody, Unconjugated (bs-2072R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.