bs-20745R [Primary Antibody]
Nurr1 Polyclonal Antibody
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Host: Rabbit

Target Protein: Nurr1

Immunogen Range: 111-200/598


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4929

Swiss Prot: P43354

Source: KLH conjugated synthetic peptide derived from human Nurr1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Nuclear receptor and transcription factor; plays a role in development and maintenance of neurons synthesizing the neurotransmitter dopamine This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson disease, schizophernia, and manic depression. Misregulation of this gene may be associated with rheumatoid arthritis. Four transcript variants encoding four distinct isoforms have been identified for this gene. Additional alternate splice variants may exist, but their full length nature has not been determined.

Size: 100µL

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(ICC)(1:50-200)
IF(1:50-200)

Predicted Molecular Weight: 67 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Nurr1 Polyclonal Antibody, Unconjugated (bs-20745R) at 1:400 overnight at 4°C, DAB staining.


Blank control:Hela. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody (bs-20745R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Protocol Hela cells were  incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Nurr1 Antibody(bs-20745R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).