bs-20745R-FITC [Conjugated Primary Antibody]
Nurr1 Polyclonal Antibody, FITC Conjugated
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Host: Rabbit

Target Protein: Nurr1

Immunogen Range: 111-200/598


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4929

Swiss Prot: P43354

Source: KLH conjugated synthetic peptide derived from human Nurr1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Nuclear receptor and transcription factor; plays a role in development and maintenance of neurons synthesizing the neurotransmitter dopamine This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson disease, schizophernia, and manic depression. Misregulation of this gene may be associated with rheumatoid arthritis. Four transcript variants encoding four distinct isoforms have been identified for this gene. Additional alternate splice variants may exist, but their full length nature has not been determined.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100µL

Concentration: 1ug/ul

Applications: FCM(1:20-100)
IF(ICC)(1:50-200)
IF(1:50-200)

Predicted Molecular Weight: 67 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Hela cells were fixed with 4% PFA for 10min at room temperature，permeabilized with 90% ice-cold methanol for 20 min at -20℃,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Nurr1 Polyclonal Antibody, FITC Conjugated（bs-20745R-FITC） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).


HepG2 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Nurr1 Polyclonal Antibody(bs-20745R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Blank control:Hela. Primary Antibody (green line): Rabbit Anti-Nurr1 antibody (bs-20745R-FITC) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Protocol Hela cells were  incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained withNurr1 Antibody(bs-20745R-FITC)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).