bs-20747R [Primary Antibody]
GPR49/LGR5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GPR49/LGR5

Immunogen Range: 211-310/907


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 25406

Swiss Prot: O75473

Source: KLH conjugated synthetic peptide derived from human GPR49/LGR5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Main cell surface receptor for hyaluronate. Adhesion to ucosal high endothelial venule and to types I and VI collagen. robably involved in matrix adhesion, lymphocyte activation and ymph node homing.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:50-200)
IHC-F(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 98


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu Yahong. et al. Preclinical Evaluation of Safety, Pharmacokinetics, Efficacy, and Mechanism of Radioprotective Agent HL-003. Oxid Med Cell Longev. 2021;2021:6683836Read more>>
  • Chaojie Li. et al. Hypoxia-induced Tie1 drives stemness and cisplatin resistance in non-small cell lung carcinoma cells. Cancer Cell Int. 2021 Dec;21(1):1-12Read more>>
  • Ling Ding. et al. Bufalin Inhibits Tumorigenesis, Stemness, and Epithelial–Mesenchymal Transition in Colorectal Cancer through a C-Kit/Slug Signaling Axis. INT J MOL SCI. 2022 Jan;23(21):13354Read more>>
  • Qi Yadong. et al. Heat-inactivated Bifidobacterium adolescentis ameliorates colon senescence through Paneth-like-cell-mediated stem cell activation. NAT COMMUN. 2023 Sep;14(1):1-19Read more>>
  • Chengcheng Li. et al. LGR5, a prognostic stem cell target, promotes endometrial cancer proliferation through autophagy activation. TRANSL ONCOL. 2024 Feb;40:101853Read more>>
VALIDATION IMAGES

Lane 1: Mouse Brain lysates; Lane 2: Rat Brain lysates probed with LGR5 Polyclonal Antibody, Unconjugated (bs-20747R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse Cerebrum ysates; Lane 2: Mouse Stomach lysates; Lane 3: Mouse Colon lysates probed with GPR49/LGR5 Polyclonal Antibody, Unconjugated (bs-20747R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.