bs-20750R [Primary Antibody]
UCP-2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: UCP-2

Immunogen Range: 121-220/309


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7351

Swiss Prot: P55851

Source: KLH conjugated synthetic peptide derived from human UCP-2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. UCP2 gene is expressed in many tissues, with the greatest expression in skeletal muscle. UCP2 is thought to play a role in non shivering thermogenesis, obesity and diabetes.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 34


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jianwei Wang. et al. A Preliminary Study on the Effect of Hydrogen Gas on Alleviating Early CCl4-Induced Chronic Liver Injury in Rats. Antioxidants-Basel. 2021 Dec;10(12):1933Read more>>
  • Lei Wang. et al. Lactobacillus plantarum DP189 Reduces _-SYN Aggravation in MPTP-Induced Parkinsons Disease Mice via Regulating Oxidative Damage, Inflammation, and Gut Microbiota Disorder. J Agr Food Chem. 2022;70(4):11631173Read more>>
  • Li Xu. et al. Fenpropathrin increases gliquidone absorption via causing damage to the integrity of intestinal barrier. ECOTOX ENVIRON SAFE. 2022 Sep;242:113882Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with UCP-2 Polyclonal Antibody, Unconjugated (bs-20750R) at 1:400 overnight at 4°C, DAB staining.


Hela cell lysates probed with UCP-2 Polyclonal Antibody, Unconjugated (bs-20750R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Stomach lysates; Lane 2: Mouse Spleen lysates; Lane 3: Mouse NIH/3T3 cell lysates; Lane 4: Hhuman DU145 cell lysates probed with UCP-2 Polyclonal Antibody, Unconjugated (bs-20750R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.