bs-20773R-A555 [Conjugated Primary Antibody]
Caspase-9 Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Caspase-9

Immunogen Range: 231-330/416


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 842

Swiss Prot: P55211

Source: KLH conjugated synthetic peptide derived from human Caspase-9

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin.?

Conjugation: ALEXA FLUOR® 555

Excitation/ Emission: 555nm/580nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 35/50


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Sheep
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Muhammad T et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020 Feb 22. Read more>>
  • Xiaoli Gao. et al. Effects of Clostridium perfringens beta2 toxin on apoptosis, inflammation, and barrier function of intestinal porcine epithelial cells. Microb Pathogenesis. 2020 Oct;147:104379Read more>>
  • Furui Han. et al. In vivo and in vitro study on hepatotoxicity of Tris-(2, 3-dibromopropyl) isocyanurate exposure via mitochondrial and death receptor pathway. ECOTOX ENVIRON SAFE. 2022 Nov;246:114186Read more>>
  • Zhu Sai-ya. et al. COX17 restricts renal fibrosis development by maintaining mitochondrial copper homeostasis and restoring complex IV activity. ACTA PHARMACOL SIN. 2023 May;:1-12Read more>>
  • Lisha Peng. et al. miR-128-3p increases the radiosensitivity in nasopharyngeal carcinoma via regulating vascular endothelial growth factor C. PATHOL RES PRACT. 2023 Aug;:154768Read more>>
  • Cuicui Zhuang. et al. Escherichia coli infection mediates pyroptosis via activating p53-p21 pathway-regulated apoptosis and cell cycle arrest in bovine mammary epithelial cells. MICROB PATHOGENESIS. 2023 Sep;:106338Read more>>
  • ohan Chen. et al. Inhalation of ammonia promotes apoptosis and induces autophagy in hepatocytes via Bax/BCl-2 and m-TOR/ATG5/LC-3bII axes. SCI TOTAL ENVIRON. 2024 Feb;912:169036Read more>>
VALIDATION IMAGES