bs-2081R [Primary Antibody]
TNF alpha Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TNF alpha

Immunogen Range: 181-235/235

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 21926

Swiss Prot: P06804

Source: KLH conjugated synthetic peptide derived from mouse TNF-alpha

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months..

Background: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Cross Reactive Species: Human
Mouse
Rat
Rabbit
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Morishita, Seita, et al. ʺSystemic Simvastatin Rescues Retinal Ganglion Cells from Optic Nerve Injury Possibly through Suppression of Astroglial NF-κB Activation.ʺ PloS one 9.1 (2014): e84387.Read more>>
  • Wang, Shan, et al. "5-Aminolevulinic Acid–mediated Sonodynamic Therapy Reverses Macrophage and Dendritic Cell Passivity in Murine Melanoma Xenografts." Ultrasound in Medicine & Biology (2014).Read more>>
  • Sun, Chao, et al. "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." BioMed Research International 2015 (2015).Read more>>
  • Wang, Yi, et al. "Targeted knockout of TNF‑α by injection of lentivirus‑mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats." Molecular Medicine Reports.Read more>>
  • Xu, Qiang, et al. "Role of Dectin-1 in the innate immune response of rat corneal epithelial cells to Aspergillus fumigatus." BMC Ophthalmology 15.1 (2015): 126.Read more>>
  • Yue, Yuan, et al. "Synergistic inhibitory effects of naproxen in combination with magnolol on TPA-induced skin inflammation in mice." RSC Advances6.44 (2016): 38092-38099.Read more>>
  • Ji, Mingli, et al. "Protective effect of Xuebijing injection against acute lung injury induced by left ventricular ischemia/reperfusion in rabbits."Experimental and Therapeutic Medicine 12.1 (2016): 51-58.Read more>>
  • Shi, Xiaowei, et al. "Panax notoginseng saponins administration modulates pro-/anti-inflammatory factor expression and improves neurologic outcome following permanent MCAO in rats." Metabolic Brain Disease (2016): 1-13.Read more>>
  • Du, Jinghua, et al. "TLR4‑dependent signaling pathway modulation: A novel mechanism by which pioglitazone protects against nutritional fibrotic steatohepatitis in mice." Molecular medicine reports 13.3 (2016): 2159-2166.Read more>>
  • Dong, Min, et al. "AG1296 enhances plaque stability via inhibiting inflammatory responses and decreasing MMP-2 and MMP-9 expression in ApoE−/− mice." Biochemical and Biophysical Research Communications (2017).Read more>>
  • Han, Sheng, et al. "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." Journal of Experimental & Clinical Cancer Research 36.1 (2017): 83.Read more>>
  • Wang, Xinhong, et al. "Hepatocyte growth factor (HGF) optimizes oral traumatic ulcer healing of mice by reducing inflammation." Cytokine (2017).Read more>>
  • Fang et al. Ischemic preconditioning protects against spinal cord ischemia-reperfusion injury in rabbits by attenuating blood spinal cord barrier disruption. (2013) Int.J.Mol.Sc. 14:10343-54Read more>>
  • Luo et al. Maternal Bisphenol A Diet Induces Anxiety-like Behavior in Female Juvenile with Neuroimmune Activation. (2014) Toxicol.Sc. Read more>>
  • Wang et al. Inhibitory effect of Zanthoxylum bungeanum seed oil on ovalbumin?induced lung inflammation in a murine model of asthma. (2016) Mol.Med.Rep. 13:4289-302Read more>>
  • Bertuglia et al. Pro-inflammatory cytokines and structural biomarkers are effective to categorize osteoarthritis phenotype and progression in Standardbred racehorses over five years of racing career. (2016) BMC.Vet.Res. 12:246Read more>>
  • Lennikov et al. Selective IKK2 inhibitor IMD0354 disrupts NF-κB signaling to suppress corneal inflammation and angiogenesis. (2018) Angiogenesis. Read more>>
  • Li et al. Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation. (2017) Mol.Med.Rep. 16:4455-4462Read more>>
  • Qian et al. FSH aggravates bone loss in ovariectomised rats with experimental periapical periodontitis. (2016) Mol.Med.Rep. 14:2997-3006Read more>>
  • Ding,et al.ZEB2 Attenuates LPS-Induced Inflammation by the NF-κB Pathway in HK-2 Cells.(2018) Inflammation. 41:722-731.Read more>>
  • Kim,et al.Local administration of nuclear factor of activated T cells (NFAT) c1 inhibitor to suppress early resorption and inflammation induced by bone morphogenetic protein-2.(2018) Journal of Biomedical Materials Research. Part A. 106:1299-1310.Read more>>
  • Wang,et al.Cx43 and NMDA receptors changes in UL122 genetically modified mice hippocampus: a mechanism for spatial memory impairment.(2018) International Journal of Clinical and Experimental Pathology. 11:129-1379.Read more>>
  • Luo Y et al. MCC950 attenuated early brain injury by suppressing NLRP3 inflammasome after experimental SAH in rats. Brain Res Bull. 2019 Mar;146:320-326. Read more>>
  • Jena PK et al. Long-term Western diet intake leads to dysregulated bile acid signaling and dermatitis with Th2 and Th17 pathway features in mice. J Dermatol Sci. 2019 Jun 8. pii: S0923-1811(19)30145-8.Read more>>
  • Hou DD et al. Anti-inflammatory effects of quercetin in a mouse model of MC903-induced atopic dermatitis. Int Immunopharmacol. 2019 Jun 7;74:105676.Read more>>
  • Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.Read more>>
  • Zhang J et al. Bradykinin contributes to immune liver injury via B2R receptor-mediated pathways in trichloroethylene sensitized mice: a role in Kupffer cell activation. Toxicology. 2019 Mar 1;415:37-48.Read more>>
  • Ma WJ et al. Biliary antibiotics irrigation for E. coli-induced chronic proliferative cholangitis and hepatolithiasis: A pathophysiological study in rabbits. Clin Res Hepatol Gastroenterol. 2019 Aug 13. pii: S2210-7401(19)30173-1.Read more>>
  • Gao X et al. Inhibition of LOX-1 prevents inflammation and photoreceptor cell death in retinal degeneration. Int Immunopharmacol. 2020 Mar;80:106190. Read more>>
VALIDATION IMAGES

Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated (bs-2081R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.