Host: Rabbit
Target Protein: TNF alpha
Immunogen Range: 181-235/235
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 21926
Swiss Prot: P06804
Source: KLH conjugated synthetic peptide derived from mouse TNF-alpha
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Storage Condition: Store at -20°C for 12 months..
Background: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-1000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Cross Reactive Species:
Human
Mouse
Rat
Rabbit
Horse
For research use only. Not intended for diagnostic or therapeutic use.
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Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated (bs-2081R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.