bs-2090R [Primary Antibody]
P53 Polyclonal Antibody
www.biossusa.com
support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: P53

Immunogen Range: 301-393/393


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7157

Swiss Prot: P04637

Source: KLH conjugated synthetic peptide derived from human P53

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 53


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Yang et al. Effect of saw palmetto extract on PI3K cell signaling transduction in human glioma. (2014) Exp.Ther.Me. 8:563-566Read more>>
  • Yang et al. RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells. (2011) J.Biol.Che. 286:11865-74Read more>>
  • Chen et al. STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7. (2015) Cancer.Cell.Int. 15:111Read more>>
  • Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203.Read more>>
  • Yang,et al.GP73 promotes invasion and metastasis of bladder cancer by regulating the epithelial-mesenchymal transition through the TGF-β1/Smad2 signalling pathway.(2018) Journal of Cellular and Molecular Medicine. 22:1650-1665.Read more>>
  • Colas,et al.Impaired Production and Diurnal Regulation of Vascular RvDn-3 DPA Increase Systemic Inflammation and Cardiovascular Disease.(2018) Circulation Research. 122:855-863.Read more>>
  • Gao D et al. Protective effect of astaxanthin against contrast-induced acute kidney injury via SIRT1-p53 pathway in rats.(2018)Int Urol NephrolRead more>>
  • Zhang Y et al. Comparative study on seasonal hair follicle cycling by analysis of the transcriptomes from cashmere and milk goats. Genomics. 2019 Feb 16. pii: S0888-7543(18)30576-7. Read more>>
  • Zhao Y et al. Walnut Polyphenol Extract Protects against Malathion- and Chlorpyrifos-Induced Immunotoxicity by Modulating TLRx-NOX-ROS. Nutrients. 2020 Feb 27;12(3). pii: E616. Read more>>
  • Danlu Yang. et al. -elemene promotes the senescence of glioma cells through regulating YAP-CDK6 signaling. Am J Cancer Res. 2021; 11(2): 370C388Read more>>
  • Xingxing Xu. et al. D-galactose induces senescence of glioblastoma cells through YAP-CDK6 pathway. Aging-Us. 2020 Sep 30; 12(18): 18501C18521Read more>>
  • Zihao Feng. et al. Bioinformatics and experimental findings reveal the therapeutic actions and targets of pachymic acid against cystitis glandularis. 2021 Apr 24Read more>>
  • Zixuan Liu. et al. Iron-bearing nanoparticles trigger human umbilical vein endothelial cells ferroptotic responses by promoting intracellular iron level. Environ Pollut. 2021 Oct;287:117345Read more>>
  • Yan Zhang. et al. Polysaccharide from Ganoderma lucidum ameliorates cognitive impairment by regulating the inflammation of the brainCliver axis in rats. 2021 May 18Read more>>
  • Xingxing Xu. et al. YAP prevents premature senescence of astrocytes and cognitive decline of Alzheimer's disease through regulating CDK6 signaling. 2021 Aug 2Read more>>
  • Yu, Ting-Ting. et al. Harnessing chlorin e6 loaded by functionalized iron oxide nanoparticles linked with glucose for target photodynamic therapy and improving of the immunogenicity of lung cancer. J Cancer Res Clin. 2022 Jan;:1-13Read more>>
  • Ning Han. et al. Ferroptosis triggered by dihydroartemisinin facilitates chlorin e6 induced photodynamic therapy by inhibiting GPX4 and enhancing ROS. Eur J Pharmacol. 2022 Feb;:174797Read more>>
  • Yu TT. et al. Chlorin e6-Induced Photodynamic Effect Polarizes the Macrophage Into an M1 Phenotype Through Oxidative DNA Damage and Activation of STING.. Front Pharmacol. 2022 Mar;13:837784-837784Read more>>
  • Yang, Xiao-Xin. et al. A nanoreactor boosts chemodynamic therapy and ferroptosis for synergistic cancer therapy using molecular amplifier dihydroartemisinin. J NANOBIOTECHNOL. 2022 Dec;20(1):1-19Read more>>
  • Liu-Gen Li. et al. Dihydroartemisinin remodels macrophage into an M1 phenotype via ferroptosis-mediated DNA damage. FRONT PHARMACOL. 2022; 13: 949835Read more>>
  • Ning Han. et al. Dihydroartemisinin elicits immunogenic death through ferroptosis-triggered ER stress and DNA damage for lung cancer immunotherapy. PHYTOMEDICINE. 2023 Jan;:154682Read more>>
  • Ting-Ting Yu. et al. Chlorin e6-induced photodynamic effect facilitates immunogenic cell death of lung cancer as a result of oxidative endoplasmic reticulum stress and DNA damage. INT IMMUNOPHARMACOL. 2023 Feb;115:109661Read more>>
  • Qi-Rui Li. et al. Laser-triggered intelligent drug delivery and anti-cancer photodynamic therapy using platelets as the vehicle. PLATELETS. 2023;34(1):Article: 2166677Read more>>
  • Yin Yang. et al. Toosendanin-induced apoptosis in canine mammary tumor CMT-U27 cells is mediated through the mitochondrial apoptotic pathway. VET COMP ONCOL. 2023 FebRead more>>
  • Yuan-Jian Hui. et al. Up-regulation of ABCG2 by MYBL2 deletion drives Chlorin e6-mediated photodynamic therapy resistance in colorectal cancer. PHOTODIAGN PHOTODYN. 2023 Apr;:103558Read more>>
  • Liu-Gen Li. et al. A Dihydroartemisinin-Loaded Nanoreactor Motivates Anti-Cancer Immunotherapy by Synergy-Induced Ferroptosis to Activate Cgas/STING for Reprogramming of Macrophage. ADV HEALTHC MATER. 2023 Aug;:2301561Read more>>
  • Qiuxiang Cai. et al. Pasteurella multocida causes liver injury in ducks by mediating inflammatory, apoptotic and autophagic pathways. MICROB PATHOGENESIS. 2023 Sep;:106336Read more>>
  • Zi-Yi Yang. et al. Cepharanthine synergizes with photodynamic therapy for boosting ROS-driven DNA damage and suppressing MTH1 as a potential anti-cancer strategy. PHOTODIAGN PHOTODYN. 2023 Nov;:103917Read more>>
  • Xue Zhang. et al. Homoharringtonine induces apoptosis of mammary carcinoma cells by inhibiting the AKT/mTOR signaling pathway. VET COMP ONCOL. 2023 DecRead more>>
  • Hui Yuan-Jian. et al. B-Myb deficiency boosts bortezomib-induced immunogenic cell death in colorectal cancer. SCI REP-UK. 2024 Apr;14(1):1-15Read more>>
  • Li Liu-Gen. et al. Dihydroartemisinin-driven selective anti-lung cancer proliferation by binding to EGFR and inhibition of NRAS signaling pathway-induced DNA damage. SCI REP-UK. 2024 May;14(1):1-15Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Anti-P53(wt-p53) Polyclonal Antibody, Unconjugated (bs-2090R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


25 ug total protein per lane of various lysates (see on figure) probed with P53 polyclonal antibody, unconjugated (bs-2090R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (P53) polyclonal Antibody, unconjugated (bs-2090R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.