Host: Rabbit
Target Protein: TEM1/CD248
Immunogen Range: 251-350/757
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 57124
Swiss Prot: Q9HCU0
Source: KLH conjugated synthetic peptide derived from human TEM 1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 80
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Pig
Horse
For research use only. Not intended for diagnostic or therapeutic use.
- Farinon, Mirian, et al. "Disease modifying anti-rheumatic activity of the alkaloid montanine on experimental arthritis and fibroblast-like synoviocytes." European Journal of Pharmacology (2017).Read more>>
- Clarimundo, Vanessa Schuck, et al. "Gastrin-releasing peptide and its receptor increase arthritis fibroblast-like synoviocytes invasiveness through activating the PI3K/AKT pathway." Peptides (2017).Read more>>
- Taguchi K et al. Isolation of tumor endothelial cells from murine cancer. (2018)Journal of Immunological Methods. Nov 3.Read more>>
- Tao Xu. et al. CD248 as a novel therapeutic target in pulmonary arterial hypertension. Clin Transl Med. 2020 Sep;10(5):e175Read more>>
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Rabbit Anti-TEM1/CD248 Polyclonal Antibody (bs-2101R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Rabbit Anti-TEM1/CD248 Polyclonal Antibody (bs-2101R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Human 293T cells probed with Rabbit Anti-TEM1/CD248 Polyclonal Antibody, Unconjugated (bs-2101R) (green) at 1:50 for 40 minutes followed by a conjugated secondary compared to control cells (blue).
Mouse spleen lysates probed with TEM1 Polyclonal Antibody, unconjugated (bs-2101R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Mouse thymus lysates probed with TEM1 Polyclonal Antibody, unconjugated (bs-2101R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Mouse small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TEM1/CD248 Polyclonal Antibody, Unconjugated (bs-2101R) at 1:400 overnight at 4°C, followed by a FITC conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TEM1/CD248 Polyclonal Antibody, Unconjugated (bs-2101R) at 1:400 overnight at 4°C, DAB staining.