bs-2116R [Primary Antibody]
E Tag Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: E Tag

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated E Tag synthetic peptide (GAPVPYPDPLEPR)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

E Tag is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Cross Reactive Species: Tags
Others
(Recombinant fusion protein, E tag)

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Lin-Xu, et al. "Escherichia coli surface display of single-chain antibody VRC01 against HIV-1 infection." Virology 475 (2015): 179-186.Read more>>
  • Wang, Lin-Xu, et al. "Escherichia coli surface display of single-chain antibody VRC01 against HIV-1 infection." Virology 475 (2015): 179-186.Read more>>
VALIDATION IMAGES

Lane 1: E.coli Lysate lysates probed with E Tag Polyclonal Antibody, Unconjugated (bs-2116R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.