bs-2130R-RBITC [Conjugated Primary Antibody]
Ki-67 Polyclonal Antibody, RBITC Conjugated
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Host: Rabbit

Target Protein: Ki-67

Immunogen Range: 1201-1300/3256


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4288

Swiss Prot: P46013

Source: KLH conjugated synthetic peptide derived from human Ki67

Purification: purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Proliferation Marker Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Conjugation: RBITC

Excitation/ Emission: 570nm/595nm

Size: 100ul

Concentration: 1mg/ml

Applications: IHC-F(1:200-800)
IF(1:200-800)
IHC-P(1:200-800)
ICC/IF(1:100-500)
WB(1:500-2000)
Flow-Cyt(1ug/Test)

Predicted Molecular Weight: 358 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
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  • Li, Ning, et al. "Amphiphilic peptide dendritic copolymer-doxorubicin nanoscale conjugate self-assembled to enzyme-responsive anti-cancer agent." Biomaterials (2014).Read more>>
  • Luo, Xiaoming, et al. "Antimetastasis and antitumor efficacy promoted by sequential release of vascular disrupting and chemotherapeutic agents from electrospun fibers." International Journal of Pharmaceutics (2014).Read more>>
  • Pan, Dayi, et al. "PEGylated dendritic diaminocyclohexyl-platinum (II) conjugates as pH-responsive drug delivery vehicles with enhanced tumor accumulation and antitumor efficacy." Biomaterials (2014).Read more>>
  • Zhao, Yuechao, et al. "Estrogen-Induced CCN1 is Critical for Establishment of Endometriosis-like Lesions in Mice." Molecular Endocrinology (2014).Read more>>
  • Yuan, Qing, et al. "Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity." International Journal of Nanomedicine 9 (2014): 4829.Read more>>
  • Yang, Fangfang, et al. "Hydroxysafflor yellow A inhibits angiogenesis of hepatocellular carcinoma via blocking ERK/MAPK and NF-κB signaling pathway in H22 tumor-bearing mice." European Journal of Pharmacology(2015).Read more>>
  • Zhang, Jing-Yao, et al. "Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice." World Journal of Gastroenterology 21.14 (2015): 4195-4209.Read more>>
  • Olianas, Maria C., Simona Dedoni, and Pierluigi Onali. "Antidepressants activate the lysophosphatidic acid receptor LPA 1 to induce insulin-like growth factor-I receptor transactivation, stimulation of ERK1/2 signaling and cell proliferation in CHO-K1 fibroblasts." Biochemical Pharmacology (2015).Read more>>
  • Luo, Xiaoming, et al. "Pharmacokinetics and antitumor efficacy of micelles assembled from multiarmed amphiphilic copolymers with drug conjugates in comparison with drug-encapsulated micelles." European Journal of Pharmaceutics and Biopharmaceutics (2015).Read more>>
  • Zhao, Yuechao, et al. "Multiple Beneficial Roles of Repressor of Estrogen Receptor Activity (REA) in Suppressing the Progression of Endometriosis."Endocrinology (2016): en-2015.Read more>>
  • Ma, Wei‑Yuan, Kun Jia, and Yan Zhang. "IL-17 promotes keratinocyte proliferation via the downregulation of C/EBPα." Experimental and Therapeutic Medicine.Read more>>
  • Antognelli, Cinzia, et al. "Glyoxalase I drives epithelial-to-mesenchymal transition via argpyrimidine-modified Hsp70, miR-21 and SMAD signalling in human bronchial cells BEAS-2B chronically exposed to crystalline silica Min-U-Sil 5: transformation into a neoplastic-like phenotype." Free Radical Biology and Medicine (2016).Read more>>
  • Li, Xin, Xiumei Zhu, and Liyan Qiu. "Constructing aptamer anchored nanovesicles for enhanced tumor penetration and cellular uptake of water soluble chemotherapeutics." Acta Biomaterialia (2016).Read more>>
  • Sondag, Gregory R. OSTEOACTIVIN AND CD44: A NOVEL INTERACTION REGULATING BONE CELL DIFFERENTIATION AND FUNCTION. Diss. Kent State University, 2015.Read more>>
  • Aslaner, Arif, et al. "Medical ozone treatment ameliorates the acute distal colitis in rat." Acta Cirurgica Brasileira 31.4 (2016): 256-263.Read more>>
  • Drel, Viktor R., et al. "Centrality of bone marrow in the severity of gadolinium-based contrast-induced systemic fibrosis." The FASEB Journal(2016): fj-201500188R.Read more>>
  • Thompson, Christopher, et al. "Loss of caveolin-1 alters extracellular matrix protein expression and ductal architecture in murine mammary glands." PLOS ONE 12.2 (2017): e0172067.Read more>>
  • He, Nan, et al. "Tumor pH-responsive Release of Drug-conjugated Micelles from Fiber Fragments for Intratumoral Chemotherapy." ACS Applied Materials & Interfaces (2017).Read more>>
  • Liang, Guodong, et al. "Silencing of CEMIP suppresses Wnt/β-catenin/Snail signaling transduction and inhibits EMT program of colorectal cancer cells." Acta Histochemica (2017).Read more>>
  • Ma et al. IL-17 promotes keratinocyte proliferation via the downregulation of C/EBPα. (2016) Exp.Ther.Me. 11:631-636Read more>>
  • Dong et al. YY1 promotes HDAC1 expression and decreases sensitivity of hepatocellular carcinoma cells to HDAC inhibitor. (2017) Oncotarget. 8:40583-40593Read more>>
  • Wu et al. A retrospective, single-center cohort study on 65 patients with primary retroperitoneal liposarcoma. (2018) Oncol.Lett. 15:1799-1810Read more>>
  • Cuellar et al. Immunohistochemical Localization of Bone Morphogenetic Proteins (BMPs) and their Receptors in Solitary and Multiple Human Osteochondromas. (2014) J.Histochem.Cytochem. 62:488-498Read more>>
  • Chen et al. Silencing of Paternally Expressed Gene 10 Inhibits Trophoblast Proliferation and Invasion. (2015) PLoS.One. 10:e0144845Read more>>
  • Li et al. Visfatin Destabilizes Atherosclerotic Plaques in Apolipoprotein E-Deficient Mice. (2016) PLoS.One. 11:e0148273Read more>>
  • Zheng et al. The Modification of Tet1 in Male Germline Stem Cells and Interact with PCNA, HDAC1 to promote their Self-renewal and Proliferation. (2016) Sci.Rep. 6:37414Read more>>
  • Luo M et al. LncRNA CASC2 inhibits proliferation and migration of adenocarcinoma cells via miR-4735-3p and mTOR.(2018)J. Cell. Biochem.Nov 18. Read more>>
  • Wang A et al. LEF1-AS1 contributes to proliferation and invasion through regulating miR-544a/ FOXP1 axis in lung cancer. Invest New Drugs. 2019 Feb 8.Read more>>
  • Chen Z et al. Enzyme-powered Janus nanomotors launched from intratumoral depots to address drug delivery barriers. Chemical Engineering Journal,2019 375, 122109. Read more>>
  • Tang Y et al. LncRNA differentiation antagonizing non-protein coding RNA promotes proliferation and invasion through regulating miR-135a/NLRP37 axis in pancreatic cancer. Invest New Drugs. 2019 Jul 3. Read more>>
  • Chen Z et al. Synergistic antitumor efficacy of hybrid micelles with mitochondrial targeting and stimuli-responsive drug release. Journal of Materials Chemistry B.2019.Read more>>
  • Li T et al. Withanolides, extracted from Datura metel L. inhibit keratinocyte proliferation and imiquimod- induced psoriasis-like dermatitis via the STAT3/P38/ERK1/2 pathway. Molecules. 2019 Jul 17;24(14). pii: E2596. Read more>>
  • Li R et al. Pharmacological biotargets and the molecular mechanisms of oxyresveratrol treating colorectal cancer: Network and experimental analyses. Biofactors. 2019 Oct 24.Read more>>
  • Liu F et al. Clinical and biological significances of heat shock protein 90 (Hsp90) in human nasopharyngeal carcinoma cells and anti-cancer effects of Hsp90 inhibitor. Biomed Pharmacother. 2019 Oct 18;120:109533. Read more>>
  • Hou Y et al. SKA3 Promotes tumor growth by regulating CDK2/P53 phosphorylation in hepatocellular carcinoma. Cell Death Dis. 2019 Dec 5;10(12):929. Read more>>
  • Xie S et al. Bacteria-propelled microrockets to promote the tumor accumulation and intracellular drug uptake. Chemical Engineering Journal,2019, 123786. Read more>>
  • Zhang Q et al. The circular RNA hsa_circ_0007623 acts as a sponge of microRNA-297 and promotes cardiac repair. Biochem Biophys Res Commun. 2020 Mar 19;523(4):993-1000.Read more>>
  • Hua CD et al. Repression of Dok7 expression mediated by DNMT1 promotes glioma cells proliferation.Biomed Pharmacother. 2018 Oct;106:678-685. Read more>>
  • Jinfeng Zhu et al. Expression and prognostic significance of pyruvate dehydrogenase kinase 1 in bladder urothelial carcinoma. Virchows Arch. 2020 Nov;477(5):637-649.Read more>>
  • Jin Y et al. Oxymatrine Inhibits Renal Cell Carcinoma Progression by Suppressing -Catenin Expression. Front Pharmacol. 2020 Jun 5;11:808.Read more>>
  • Xia Qinet al. Zinc Oxide Nanoparticles Induce Ferroptotic Neuronal Cell Death in vitro and in vivo. Int J Nanomedicine. 2020 Jul 27;15:5299-5315.Read more>>
  • Di Xia et al. Nrf2 promotes esophageal squamous cell carcinoma (ESCC) resistance to radiotherapy through the CaMKII-associated activation of autophagy. Cell Biosci. 2020 Jul 30;10:90.Read more>>
  • Chengjun Sunet al. Long noncoding RNA AC092171.4 promotes hepatocellular carcinoma progression by sponging microRNA-1271 and upregulating GRB2. Aging (Albany NY). 2020 Jul 21;12(14):14141-14156.Read more>>
  • Feng Y et al. Long noncoding RNA HOXA-AS2 functions as an oncogene by binding to EZH2 and suppressing LATS2 in acute myeloid leukemia (AML)Cell Death Dis.2020 Dec 2;11(12):1025.Read more>>
  • Gong H et al. EZH2 inhibitors reverse resistance to gefitinib in primary EGFR wild-type lung cancer cellsBMC Cancer.2020 Dec 4;20(1):1189.Read more>>
  • Jingyi You. et al. Human Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Alleviate Lung Injury in Rat Model of Bronchopulmonary Dysplasia by Affecting Cell Survival and Angiogenesis. Stem Cells Dev. 2020 Nov;29(23):1520-1532Read more>>
  • Zhaohui Jia. et al. LncRNA MCM3AP-AS1 Promotes Cell Proliferation and Invasion Through Regulating miR-543-3p/SLC39A10/PTEN Axis in Prostate Cancer. Oncotargets Ther. 2020; 13: 9365C9376Read more>>
  • Liang Kong. et al. Tumor Microenvironmental Responsive Liposomes Simultaneously Encapsulating Biological and Chemotherapeutic Drugs for Enhancing Antitumor Efficacy of NSCLC. Int J Nanomed. 2020; 15: 6451C6468Read more>>
VALIDATION IMAGES