Host: Rabbit
Target Protein: Angiotensin 2 type 1A receptor
Immunogen Range: 101-200/359
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 185
Swiss Prot: P30556
Source: KLH conjugated synthetic peptide derived from human of human Angiotensin II type 1A receptor
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 41
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Liu, Junjun, et al. "Differential effects of angiotensin II receptor blockers on Aβ generation." Neuroscience Letters (2014).Read more>>
- Shimizu et al. Angiotensin II subtype 1a receptor signaling in resident hepatic macrophages induces liver metastasis formation. (2017) Cancer.Sc. 108:1757-1768Read more>>
Lane 1: Mouse liver lysates; Lane 2: Mouse lung lysate; Lane 3: mouse adrenal gland lysate probed with Angiotensin 2 type 1A receptor Polyclonal Antibody, Unconjugated (bs-2132R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Mouse kidney lysates probed with Angiotensin 2 type 1A receptor Polyclonal Antibody, Unconjugated (bs-2132R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Liver lysates; Lane 2: Mouse Heart lysates; Lane 3: Mouse Placenta lysates; Lane 4: Mouse Kidney lysates; Lane 5: Mouse Embryo lysates; Lane 6: Mouse Cerebrum lysates; Lane 7: Mouse NIH/3T3 cell lysates; Lane 8: Rat Liver lysates; Lane 9: Rat Heart lysates; Lane 10: Rat Kidney lysates; Lane 11: Rat Cerebrum lysates; Lane 12: Rat Embryo lysates probed with Angiotensin 2 type 1A receptor Polyclonal Antibody, Unconjugated (bs-2132R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated (bs-2132R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.