bs-2134R [Primary Antibody]
Thrombin light chain Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Thrombin light chain

Immunogen Range: 328-363/622


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2147

Swiss Prot: P00734

Source: KLH conjugated synthetic peptide derived from human Thrombin light chain

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 70


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sogawa, Kazuyuki, et al. "Development of a sandwich ELISA for the thrombin light chain identified by serum proteome analysis." Practical Laboratory Medicine (2017).Read more>>
  • Sogawa et al. Development of a sandwich ELISA for the thrombin light chain identified by serum proteome analysis. (2017) Pract.Lab.Med. 8:34-40Read more>>
VALIDATION IMAGES

Mouse liver lysates probed with Rabbit Anti-Thrombin light chain Polyclonal Antibody, Unconjugated (bs-2134R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.


Mouse placenta lysates probed with Rabbit Anti-Thrombin light chain Polyclonal Antibody, Unconjugated (bs-2134R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.