bs-2138R
[Primary Antibody]
PARP1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: PARP1
Immunogen Range: 201-300/1014
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 142
Swiss Prot: P09874
Source: KLH conjugated synthetic peptide derived from human PARP1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 111
Cross Reactive Species:
Human
Mouse
Rat
Chicken
Predicted Cross Reactive Species:
Dog
Cow
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Ma, Juan, et al. "A Crucial Role of Lateral Size for Graphene Oxide in Activating Macrophages and Stimulating Pro-inflammatory Responses in Cells and Animals." ACS nano (2015).Read more>>
- Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082.Read more>>
- Omar, S. S., R. G. Aly, and N. M. Badae. "Vitamin E improves testicular damage in streptozocin‐induced diabetic rats, via increasing vascular endothelial growth factor and poly (ADP‐ribose) polymerase‐1." Andrologia (2017).Read more>>
- Zhou et al. Azithromycin synergistically enhances anti-proliferative activity of vincristine in cervical and gastric cancer cells. (2012) Cancers.(Basel). 4:1318-32Read more>>
- Dong, Liwei, et al. "Echinacoside Induces Apoptosis in Human SW480 Colorectal Cancer Cells by Induction of Oxidative DNA Damages." International Journal of Molecular Sciences 16.7 (2015): 14655-14668.Read more>>
- Nandi S et al. Characterization and inception of a triterpenoid astrakurkurol, as a cytotoxic molecule on human hepatocellular carcinoma cells, Hep3B. J Agric Food Chem. 2019 Jul 10;67(27):7660-7673.Read more>>
- Han S et al. FHL1 regulates myoblast differentiation and autophagy through its interaction with LC3. J Cell Physiol. 2019 Oct 21.Read more>>
- Wei J et al. Jiawei Foshou San Induces Apoptosis in Ectopic Endometrium Based on Systems Pharmacology, Molecular Docking, and Experimental Evidence. Evid Based Complement Alternat Med. 2019 Oct 27;2019:2360367. Read more>>
- Yuan P et al. The nanomaterial-induced bystander effects reprogrammed macrophage immune function and metabolic profile. Nanotoxicology. 2020 Oct;14(8):1137-1155.Read more>>
- Chen J et al. Lipoic Acid Decreases the Expression of Poly ADP-Ribose Polymerase and Inhibits Apoptosis in Diabetic Rats. Diabetes Metab Syndr Obes. 2020 May 20;13:1725-1731.Read more>>
- Gong H et al. EZH2 inhibitors reverse resistance to gefitinib in primary EGFR wild-type lung cancer cellsBMC Cancer.2020 Dec 4;20(1):1189.Read more>>
- Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12Read more>>
- Lijin Guo. et al. Whole Transcriptome Analysis of Chicken Bursa Reveals Candidate Gene That Enhances the Hosts Immune Response to Coccidiosis. Front Physiol. 2020; 11: 573676Read more>>
- Jinjiang Yang. et al. Platelet-rich plasma attenuates interleukin-1-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2. Tissue Cell. 2021 Sep;:101646Read more>>
- Luya Pu. et al. Icariin arrests cell cycle progression and induces cell apoptosis through the mitochondrial pathway in human fibroblast-like synoviocytes. Eur J Pharmacol. 2021 Dec;912:174585Read more>>
- Sudeshna Nandi. et al. Anti-cancer effect of astrakurkurol from a folklore tribal mushroom on human hepatocellular carcinoma cells via mediating cell cycle inhibition, apoptosis, and migration. 2021 Nov 22Read more>>
- Zhihai Yu. et al. Two contradictory facades of N-acetylcysteine activity towards renal carcinoma cells. J TAIBAH UNIV SCI. 2022;16(1):423-431Read more>>
- Nanqing Jing. et al. Both live and heat-killed Bifidobacterium animalis J-12 alleviated oral ulcers in LVG golden Syrian hamsters by gavage by directly intervening in the intestinal flora structure. FOOD FUNCT. 2023 JaRead more>>
VALIDATION IMAGES
Human HeLa lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Human Raji lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Formalin-fixed and paraffin embedded rat brain labeled with Anti PARP (N-Terminus) Polyclonal Antibody, Unconjugated(bs-2138R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C and DAPI
Lane 1: Rat Brain; Probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:1000 in 4˚C.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Hela Cells; Lane 2: Raji Cells; Lane 3: HepG2 Cells; Lane 4: K562 Cells; 30ug loaded into the lane; Probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody at 1:20000 for 60 minutes at 37°C. Lower band is the cleaved PARP1 form.
HL-60 cells were fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were incubated in 1XPBS/2%BSA/10% goat serum for 15 min at room temperature to block non-specific protein-protein interactions. Next, the cells were probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:500 (0.2ug/1x10^6 Cells) for 30 minutes at room temperature, followed by a Goat anti-rabbit IgG-PE (bs-0295G-PE) secondary antibody for 40 mins at room temperature. Acquisition of 20,000 events was performed. Primary Antibody staining (green) is compared to unstained cells (dark blue), secondary only (light blue) and isotype control (bs-0295P; orange).
Lane 1: Human 293T cell lysates; Lane 2: Human HL-60 cell lysates; Lane 3: Human K562 cell lysates; Lane 4: Human SH-SY5Y cell lysates; Lane 5: Human U251 cell lysates; Lane 6: Human Hela cell lysates probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.