bs-2150R-BF350 [Conjugated Primary Antibody]
TNF-alpha Polyclonal Antibody, AbBy Fluor® 350 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: TNF-alpha

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 403922

Source: KLH conjugated synthetic peptide derived from dog TNF-alpha

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS PAGE under non reducing conditions. TNF alpha is closely related to the 25 kDa protein Tumour Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Conjugation: AbBy Fluor® 350

Excitation/ Emission: 346nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Dog
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhang, Jing-Jing, et al. "“Proof-of-principle” concept for ultrasensitive detection of cytokines based on the electrically heated carbon paste electrode." Chemical Communications 47.23 (2011): 6551-6553.Read more>>
  • Liao, Peng, et al. "Eccentric contraction induces inflammatory responses in rat skeletal muscle: role of tumor necrosis factor-α." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 298.3 (2010): R599-R607.Read more>>
  • Wu et al. TNF-a mediated inflammatory macrophage polarization contributes to the pathogenesis of steroid-induced osteonecrosis in mice. (2015) Int.J.Immunopathol.Pharmaco. 28:351-61Read more>>
  • Xi et al. Expressions of IL-6, TNF-α and NF-κB in the skin of Chinese brown frog (Rana dybowskii). (2017) Eur.J.Histochem. 61:2834Read more>>
  • Hu Y et al.Mechanical stretch aggravates aortic dissection by regulating MAPK pathway and the expression of MMP-9 and inflammation factors.(2018) Biomedicine & Pharmacotherapy, 108, 1294–1302. Read more>>
  • Zhou P et al. Histamine-4 receptor antagonist JNJ7777120 inhibits pro-inflammatory microglia and prevents the progression of Parkinson-like pathology and behaviour in a rat model.(2018)Brain Behav. Immun. Nov 05.Read more>>
  • Meng H et al. Circular RNA expression profile of liver tissues in an EtOH-induced mouse model of alcoholic hepatitis. Eur J Pharmacol. 2019 Nov 5;862:172642. Read more>>
  • Guoxiu Wang. et al. Local renal complement activation mediates immune kidney injury by inducing endothelin-1 signalling and inflammation in trichloroethylene-sensitised mice. Toxicol Lett. 2020 Oct;333:13Read more>>
  • Da Zhao. et al. Exploring the Regulatory Mechanism of Nao Tai Fang on Vascular Dementia's Biological Network Based on Cheminformatics and Transcriptomics Strategy. J Ethnopharmacol. 2021 Mar;:114065Read more>>
  • Yanhong Li. et al. Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model. Stem Cell Res Ther. 2021 Dec;12(1):1-14Read more>>
  • Claudia C. Cotomacio. et al. Wound healing process with different photobiomodulation therapy protocols to treat 5-FU-induced oral mucositis in hamsters. Arch Oral Biol. 2021 Nov;131:10525Read more>>
  • Zhou, Can-Can. et al. Lead Exposure in Developmental Ages Promotes A Accumulation by Disturbing A Transportation in Blood-Cerebrospinal Fluid Barrier/BloodCBrain Barriers and Impairing A Clearance in the Liver. 2021 Nov 17Read more>>
  • Juin-Hong Cherng. et al. Hemostasis and Anti-Inflammatory Abilities of AuNPs-Coated Chitosan Dressing for Burn Wounds. J PERS MED. 2022 Jul;12(7):1089Read more>>
  • Yanhong Li. et al. Allogeneic Adipose-Derived Mesenchymal Stem Cell Transplantation Alleviates Atherosclerotic Plaque by Inhibiting Ox-LDL Uptake, Inflammatory Reaction and Endothelial Damage in Rabbits. CELLS-BASEL. 2023 Jan;12(15):1936Read more>>
  • Xiangjie Fu. et al. Synergistic and Long-Lasting Wound Dressings Promote Multidrug-Resistant Staphylococcus Aureus-Infected Wound Healing. INT J NANOMED. 2023 Aug 16Read more>>
  • Chengmin Feng. et al. Preparation of healing-promoting and fibrosis-inhibiting asymmetric poly(ethylene glycol-b-L-phenylalanine)/cRGD-modified hyaluronate sponges and their applications in hemorrhage and nasal mucosa repair. INT J BIOL MACROMOL. 2024 Feb;258:128911Read more>>
  • Chaoqin Shu. et al. 3D Printing of Cobalt-Incorporated Chloroapatite Bioceramic Composite Scaffolds with Antioxidative Activity for Enhanced Osteochondral Regeneration. ADV HEALTHC MATER. 2024 Feb;:2303217Read more>>
VALIDATION IMAGES