bs-21520R [Primary Antibody]
Synaptotagmin 1/SYT1 Polyclonal Antibody
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Host: Rabbit

Target Protein: Synaptotagmin 1/SYT1

Immunogen Range: 321-422/422


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6857

Swiss Prot: P21579

Source: KLH conjugated synthetic peptide derived from human Synaptotagmin 1/SYT1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS).

Size: 100µL

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 48


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Cow
Sheep
Horse
Chicken
Rabbit
Zebrafish

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Synaptotagmin 1/SYT1 Polyclonal Antibody, Unconjugated (bs-21520R) at 1:200 overnight at 4°C, DAB staining.