bs-21569R [Primary Antibody]
Brachyury Polyclonal Antibody
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Host: Rabbit

Target Protein: Brachyury

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: O15178

Swiss Prot: 6862

Source: KLH conjugated synthetic peptide derived from human Brachyury

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

he protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]

Size: 100uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

25 ug total protein per lane of various lysates (see on figure) probed with Brachyury polyclonal antibody, unconjugated (bs-21569R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.