bs-2158R [Primary Antibody]
Polycystin 2 Polyclonal Antibody
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: Polycystin 2

Specificity: This antibody may have a minor secondary cross-reactivity towards Polycystin 2-like protein 1 due to a 71% non-contiguous sequence similarity in the immunogen sequence.

Immunogen Range: 651-750/968


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5311

Swiss Prot: Q13563

Source: KLH conjugated synthetic peptide derived from human Polycystin 2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Functions as a calcium permeable cation channel involved in fluid-flow mechanosensation by the primary cilium in renal epithelium. Together with TRPV4, forms mechano- and thermosensitive channels in cilium (PubMed:18695040). PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis. Acts as a regulator of cilium length, together with PKD1. The dynamic control of cilium length is essential in the regulation of mechanotransductive signaling. The cilium length response creates a negative feedback loop whereby fluid shear-mediated deflection of the primary cilium, which decreases intracellular cAMP, leads to cilium shortening and thus decreases flow-induced signaling. Also involved in left/right axis specification downstream of nodal flow: forms a complex with PKD1L1 in cilia to facilitate flow detection in left/right patterning (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 106


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti-Polycystin 2 Polyclonal Antibody, Unconjugated (bs-2158R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.