bs-2184R [Primary Antibody]
NQO1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NQO1

Immunogen Range: 201-274/274


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1728

Swiss Prot: P15559

Source: KLH conjugated synthetic peptide derived from human NQO1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 31


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Pig
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Feng, et al. "Silymarin attenuates paraquat‐induced lung injury via Nrf2‐mediated pathway in vivo and in vitro." Clinical and Experimental Pharmacology and Physiology (2015).Read more>>
  • Liu, Cui-Zhen, et al. "HBSP attenuates lipopolysaccharide-induced inflammatory response in human renal proximal tubular epithelial cells by induction of Nrf2 via PI3K/Akt pathway." Int J Clin Exp Med 10.1 (2017): 587-597.Read more>>
  • Shen, Haitao, et al. "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." International Immunopharmacology 46 (2017): 16-22.Read more>>
  • Zhang et al. Nrf2 antioxidant pathway suppresses Numb-mediated epithelial-mesenchymal transition during pulmonary fibrosis. (2018) Cell.Death.Dis. 9:83Read more>>
  • Zhang et al. Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence. (2018) Oncotarget. 9:2175-2192Read more>>
  • Tang et al. Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways. (2016) Cell.Stress.Chaperones. 21:239-49Read more>>
  • Wang X et al. Sarcodon imbricatus polysaccharides protect against cyclophosphamide-induced immunosuppression via regulating Nrf2-mediated oxidative stress.(2018) Int. J. Biol. Macromol.Dec;120(Pt A):736-744. Read more>>
  • Han C et al. Florfenicol induces oxidative stress and hepatocyte apoptosis in broilers via Nrf2 pathway. Ecotoxicol Environ Saf. 2020 Mar 15;191:110239.Read more>>
  • Zhang Yifanet al. Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury. Oxid Med Cell Longev. 2020 Jul 9;2020:6428498.Read more>>
  • Xiao Wang. et al. Florfenicol induces renal toxicity in chicks by promoting oxidative stress and apoptosis. Environ Sci Pollut R. 2021 Jan;28(1):936-946Read more>>
  • Qingyue Yang. et al. The link between deacetylation and hepatotoxicity induced by exposure to hexavalent chromium. J Adv Res. 2021 ApRead more>>
  • Feng Gao. et al. Aucubin ameliorates the LPS\induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF\B and activating the Keap1/Nrf2 signalling pathway. 2021 Apr 27Read more>>
  • Wang, Huihui. et al. Anti-inflammatory and Antioxidant Effects of Pyrroloquinoline Quinone in L-NAME-Induced Preeclampsia-Like Rat Model. 2021 Sep 2Read more>>
  • Naoki Yoshida. et al. Relationship between Cognitive Dysfunction and Age-Related Variability in Oxidative Markers in Isolated Mitochondria of Alzheimer’s Disease Transgenic Mouse Brains. Biomedicines. 2022 Feb;10(2):281Read more>>
  • Xiao Wang. et al. Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis. Ecotox Environ Safe. 2022 Mar;233:113339Read more>>
  • Jinshan Wu. et al. Protection by Hosta ventricosa polysaccharides against oxidative damage induced by t-BHP in HepG2 cells via the JNK/Nrf2 pathway. Int J Biol Macromol. 2022 May;208:453Read more>>
  • Zhihai Yu. et al. Two contradictory facades of N-acetylcysteine activity towards renal carcinoma cells. J TAIBAH UNIV SCI. 2022;16(1):423-431Read more>>
  • Xiangyou Yu. et al. Upregulation of BRD7 protects podocytes against high glucose-induced apoptosis by enhancing Nrf2 in a GSK-3_-dependent manner. TISSUE CELL. 2022 Jun;76:101813Read more>>
  • Zhihui Liu. et al. Curcumin alleviates aristolochic acid nephropathy based on SIRT1/Nrf2/HO-1 signaling pathway. TOXICOLOGY. 2022 Sep;479:153297Read more>>
  • Yuan-yuan Wei. et al. Acute liver injury induced by carbon tetrachloride reversal by Gandankang aqueous extracts through nuclear factor erythroid 2-related factor 2 signaling pathway. ECOTOX ENVIRON SAFE. 2023 Feb;251:114527Read more>>
  • Rui Sang. et al. Taraxasterol alleviates aflatoxin B1-induced liver damage in broiler chickens via regulation of oxidative stress, apoptosis and autophagy. ECOTOX ENVIRON SAFE. 2023 Feb;251:114546Read more>>
  • Suiyi Liu. et al. Depleted uranium causes renal mitochondrial dysfunction through the ETHE1/Nrf2 pathway. CHEM-BIOL INTERACT. 2023 Feb;372:110356Read more>>
  • Chen Chunni. et al. Study on the targeted regulation of Scutellaria baicalensis leaf on glutamineCglutamate metabolism and glutathione synthesis in the liver of D-gal ageing rats. J PHARM PHARMACOL. 2023 JuRead more>>
  • Yu-Sheng Shi. et al. Dendrobine rescues cognitive dysfunction in diabetic encephalopathy by inhibiting ferroptosis via activating Nrf2/GPX4 axis. PHYTOMEDICINE. 2023 Jul;:154993Read more>>
  • Qingyu Ding. et al. N-acetylcysteine alleviates oxidative stress and apoptosis and prevents skeletal muscle atrophy in type 1 diabetes mellitus through the NRF2/HO-1 pathway. LIFE SCI. 2023 Sep;329:121975Read more>>
  • Zhiguang Zhang. et al. The Protective Effect of Marsdenia tenacissima against Cisplatin-Induced Nephrotoxicity Mediated by Inhibiting Oxidative Stress, Inflammation, and Apoptosis. MOLECULES. 2023 Jan;28(22):7582Read more>>
  • Yaqi Li. et al. Non-thermal plasma promotes boar sperm quality through increasing AMPK methylation. INT J BIOL MACROMOL. 2024 Feb;257:128768Read more>>
  • Wenting Li. et al. A collective hyperthermia-facilitated glutathione inhibition strategy for boosted oxidative stress treatment. CHEM ENG J. 2024 Apr;485:150056Read more>>
  • Xiangming Wang. et al. Protective mechanisms of Juncus effusus and Carbonized Juncus effusus against d-galactosamine-induced acute liver injury in mice. CHEM PHARM BULL. 2024 Feb 07Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse colon carcinoma labeled with Anti-NQO1 Polyclonal Antibody, Unconjugated (bs-2184R) at 1:200, followed by conjugation to the secondary antibody and DAB staining


L1 rat liver lysates L2 rat brain lysates probed with Anti NQO1 Polyclonal Antibody, Unconjugated (bs-2184R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 30kD. Observed band size:30kD.\n


Lane 1: A549 cell lysates probed with NQO1 Polyclonal Antibody, Unconjugated (bs-2184R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human Hela cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human MCF-7 cell lysates; Lane 4: Human Du145 cell lysates probed with NQO1 Polyclonal Antibody, Unconjugated (bs-2184R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NQO1) Polyclonal Antibody, Unconjugated (bs-2184R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.