bs-2188R [Primary Antibody]
GAPDH Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GAPDH

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2597

Swiss Prot: P04406

Source: GAPDH protein of rabbit

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 37


Cross Reactive Species: Human
Mouse
Rat
Bovine
Pig
Chicken
Rabbit
All

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shao, Guangcan, et al. "Construction of the plasmid coding for the expression of the EGFP‑M‑IL‑2 (88Arg, 125Ala) fusion protein and the anti‑tumor effects exerted by the fusion protein in HeLa-60 cells." Oncology Letters.Read more>>
  • Hou, Yu Sen, et al. "Lipopolysaccharide pretreatment inhibits LPS-induced human umbilical cord mesenchymal stem cell apoptosis via upregulating the expression of cellular FLICE-inhibitory protein." Molecular Medicine Reports.Read more>>
  • Zhang, Xiangliang, et al. "MicroRNA-216a enhances the radiosensitivity of pancreatic cancer cells by inhibiting beclin-1-mediated autophagy." Oncology Reports.Read more>>
  • Tan, Shifan, et al. "miR-409-3p sensitizes colon cancer cells to oxaliplatin by inhibiting Beclin-1-mediated autophagy." International Journal of Molecular Medicine.Read more>>
  • Jia, Lianqun, et al. "Effects of Tanshinone IIA on the modulation of miR‑33a and the SREBP‑2/Pcsk9 signaling pathway in hyperlipidemic rats." Molecular Medicine Reports (2016).Read more>>
  • Yan, Lijie, et al. "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." Journal of cardiovascular pharmacology 64.4 (2014): 310-317.Read more>>
  • YIN, Zhi-hong, et al. "Expression and tissue distribution analysis of Angiotensin II in sheep (Ovis aries) skins associated with white and black coat colors." Journal of Zhejiang University Science B 1 (2016).Read more>>
  • Sun, Pengchao, et al. "SL2B aptamer and folic acid dual-targeting DNA nanostructures for synergic biological effect with chemotherapy to combat colorectal cancer." International Journal of Nanomedicine 12 (2017): 2657.Read more>>
  • Wang, Hong, et al. "Osthole shows the potential to overcome P-glycoprotein‑mediated multidrug resistance in human myelogenous leukemia K562/ADM cells by inhibiting the PI3K/Akt signaling pathway." Oncology reports 35.6 (2016): 3659-3668.Read more>>
  • Yan, Lijie, et al. "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." Journal of cardiovascular pharmacology 64.4 (2014): 310-317.Read more>>
  • Steenblock et al. The Cdc42 guanine nucleotide exchange factor FGD6 coordinates cell polarity and endosomal membrane recycling in osteoclasts. (2014) J.Biol.Che. 289:18347-59Read more>>
  • Li et al. All-trans retinoic acid regulates the expression of the extracellular matrix protein fibulin-1 in the guinea pig sclera and human scleral fibroblasts. (2010) Mol.Vi. 16:689-97Read more>>
  • Xiong et al. Up-regulation of miR-138 inhibits hypoxia-induced cardiomyocyte apoptosis via down-regulating lipocalin-2 expression. (2016) Exp.Biol.Med.(Maywood. 241:25-30Read more>>
  • Zhang et al. Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence. (2018) Oncotarget. 9:2175-2192Read more>>
  • Zhang et al. Increased stathmin expression strengthens fear conditioning in epileptic rats. (2015) Biomed.Rep. 3:28-32Read more>>
  • Yang et al. Rab5-mediated VE-cadherin internalization regulates the barrier function of the lung microvascular endothelium. (2015) Cell.Mol.Life.Sci. 72:4849-66Read more>>
  • Chai et al. Hypoxia induces pulmonary arterial fibroblast proliferation, migration, differentiation and vascular remodeling via the PI3K/Akt/p70S6K signaling pathway. (2018) Int.J.Mol.Med. 41:2461-2472Read more>>
  • Huang et al. Intracellular iodinated compounds affect sodium iodide symporter expression through TSH-mediated signaling pathways. (2011) Mol.Med.Rep. 4:77-80Read more>>
  • Cao et al. Dickkopf‑3 upregulation mediates the cardioprotective effects of curcumin on chronic heart failure. (2018) Mol.Med.Rep. 17:7249-7257Read more>>
  • Zhang,et al.Interferon-γ Promotes Neuronal Repair by Transplanted Neural Stem Cells in Ischemic Rats.(2018) Stem Cells and Development. 27:355-366.Read more>>
  • Wang,et al.Screening of genes involved in epithelial-mesenchymal transition and differential expression of complement-related genes induced by PAX2 in renal tubules.() Nephrology. :.Read more>>
  • Qiu,et al.Recombinant human maspin inhibits high glucose-induced oxidative stress and angiogenesis of human retinal microvascular endothelial cells via PI3K/AKT pathway.(2018) Molecular and Cellular Biochemistry. :.Read more>>
  • Liu,et al.Cytoprotective effect and purification of novel antioxidant peptides from hazelnut (C. heterophylla Fisch) protein hydrolysates.(2018) Journal of Functional Foods. 42:203-215.Read more>>
  • Wang,et al.Chaihulonggumulitang Shows Psycho-cardiology Therapeutic Effects on Acute Myocardial Infarction by Enhancing Bone Marrow Mesenchymal Stem Cells Mobilization.(2018) Scientific Reports. 8:3724.Read more>>
  • Zhou P et al. Histamine-4 receptor antagonist JNJ7777120 inhibits pro-inflammatory microglia and prevents the progression of Parkinson-like pathology and behaviour in a rat model.(2018)Brain Behav. Immun. Nov 05.Read more>>
  • Liu et al. Surgical incision induces learning impairment in mice partially through inhibition of the brain-derived neurotrophic factor signaling pathway in the hippocampus and amygdala. (2018) Mol.Pain. 14:1744806918805902Read more>>
  • Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/10 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93.Read more>>
  • Ma W et al. A vanillin derivative suppresses the growth of HT29 cells through the Wnt/β-catenin signaling pathway. Eur J Pharmacol. 2019 Apr 15;849:43-49. Read more>>
  • Zhang X et al. BMP9 Promotes the Extracellular Matrix of Nucleus Pulposus Cells Via Inhibition of the NotchSignaling Pathway. DNA Cell Biol. 2019 Feb 13.Read more>>
  • Qian L et al. Upregulated circular RNA circ_0074027 promotes glioblastoma cell growth and invasion by regulating miR-518a-5p/IL17RD signaling pathway. Biochem Biophys Res Commun. 2019 Mar 19;510(4):515-519. Read more>>
  • Ma H et al. Interferon-γ facilitated adjuvant-induced arthritis at early stage. Scand J Immunol. 2019 Feb 9:e12757.Read more>>
  • Wang R et al. Molecular cloning and functional characterization of porcine 2',5'-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol. 2019 Mar;209:22-30.Read more>>
  • Yuan FH et al. microRNA‐30a inhibits the liver cell proliferation and promotes cell apoptosis through the JAK/STAT signaling pathway by targeting SOCS‐1 in rats with sepsis. J Cell Physiol. 2019 Apr 10. Read more>>
  • Jiang L et al. Astrocytes induce proliferation of oligodendrocyte progenitor cells via connexin 47-mediated activation of Chi3l1 expression. Eur Rev Med Pharmacol Sci. 2019 Apr;23(7):3012-3020. Read more>>
  • Liao J et al. Inhibition of Caspase-1-dependent pyroptosis attenuates copper-induced apoptosis in chicken hepatocytes.Ecotoxicol Environ Saf. 2019 Jun 15;174:110-119.Read more>>
  • Zhang H et al. Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling Pathway.Front Pharmacol. 2019 Mar 5;10:175. Read more>>
  • Wen Y et al. NADPH Oxidase Hyperactivity Contributes to Cardiac Dysfunction and Apoptosis in Rats with Severe Experimental Pancreatitis through ROS-Mediated MAPK Signaling Pathway. Oxid Med Cell Longev. 2019 May 9;2019:4578175.Read more>>
  • Jiang S et al. Inhibitory Effect of Slit2-N on VEGF165-induced proliferation of vascular endothelia via Slit2-N-Robo4-Akt pathway in choroidal neovascularization. Cell Cycle. 2019 Jun;18(11):1241-1253. Read more>>
  • Xu Q et al. Expression of macrophage migration inhibitory factor in Aspergillus fumigatus keratitis. Int J Ophthalmol. 2019 May 18;12(5):711-716. Read more>>
  • Lin S et al. Strategy for hypertrophic scar therapy: Improved delivery of triamcinolone acetonide using mechanically robust tip-concentrated dissolving microneedle array. J Control Release. 2019 May 27;306:69-82.Read more>>
  • Wang L et al. Serious Selenium Deficiency in the Serum of Patients with Kashin–Beck Disease and the Effect of Nano-Selenium on Their Chondrocytes. Biol Trace Elem Res. 2019 Jun 8. Read more>>
  • Wang XF et al. DLX5 gene regulates the Notch signaling pathway to promote glomerulosclerosis and interstitial fibrosis in uremic rats. J Cell Physiol. 2019 Jul 11.Read more>>
  • Zhang N et al. Polypeptide-engineered DNA tetrahedrons for targeting treatment of colorectal cancer via apoptosis and autophagy. J Control Release. 2019 Jul 10;309:48-58. Read more>>
  • Kaushal P et al. Ameliorative role of antioxidant supplementation on sodium-arsenite induced adverse effects on the developing rat cerebellum. Journal of Ayurveda and Integrative Medicine.2019Read more>>
  • Li H et al. Porcine 2′, 5′-oligoadenylate synthetase-like protein inhibits replication of porcine reproductive and respiratory syndrome virus. Research Square.2019.Read more>>
  • Yang F et al. Effect of Bone Morphogenetic Protein 6 (BMP6) on Chicken Granulose Cells Proliferation and Progesterone Synthesis. Brazilian Journal of Poultry Science.2019 Feb.Read more>>
  • Chen H et al. Metformin sensitized NSCLC cells to Osimertinib via AMPK‐dependent autophagy inhibition. Clin Respir J. 2019 Sep 28. Read more>>
  • Dai X et al. Silencing of MALAT1 inhibits migration and invasion by sponging miR‑1‑3p in prostate cancer cells. Mol Med Rep. 2019 Aug 22. Read more>>
  • Duan X et al. Expression of TIGIT/CD155 and correlations with clinical pathological features in human hepatocellular carcinoma. Mol Med Rep. 2019 Oct;20(4):3773-3781. Read more>>
  • Wang M et al. Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency. Glycoconj J. 2019 Sep 2. Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-2188R) at 1:400 overnight at 4°C, DAB staining.


U251 lysates probed with GAPDH Polyclonal Antibody, Unconjugated (bs-2188R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Hela lysates; Lane 2: MCF-7 lysates probed with GAPDH Polyclonal Antibody, Unconjugated (bs-2188R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Mouse muscle lysates probed with GAPDH Polyclonal Antibody, Unconjugated (bs-2188R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1:Rat Heart lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse Liver lysates probed with GAPDH Polyclonal Antibody, Unconjugated (bs-2188R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Rat muscle lysates; Lane 2: Rat heart lysates probed with GAPDH Polyclonal Antibody, Unconjugated (bs-2188R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Cerebrum lysates; Lane 4: HUVEC cell lysates ; Lane 5: A549 cell lysates;Lane 6: MCF-7 cell lysates probed with GAPDH Polyclonal Antibody, Unconjugated (bs-2188R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GAPDH Polyclonal Antibody, Unconjugated (bs-2188R) at 1:400 overnight at 4°C, DAB staining.


Lane 1: Huamn A549 cell lysates; Lane 2:Huamn U-2OS cell lysates; Lane 3: Huamn MOLT-4 cell lysates; Lane 4: Huamn A431 cell lysates probed with GAPDH Polyclonal Antibody, Unconjugated (bs-2188R) at 1:2000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human U251 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human MCF-7 cell lysates probed with GAPDH Polyclonal Antibody, Unconjugated (bs-2188R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C


Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH (Loading Control)) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH ) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH ) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH ) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH ) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH ) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH ) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH ) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH ) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH ) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAPDH ) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GAPDH) Polyclonal Antibody, Unconjugated (bs-2188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Lane 1: Mouse Cerebellum tissue lysates; Lane 2: Mouse Raw264.7 cell lysates; Lane 3: Mouse NIH/3T3 cell Lysates; Lane 4: Rat Cerebrum tissue Lysates; Lane 5: Human SH-SY5Y cell Lysates; Lane 6: Human MCF-7 cell Lysates probed with GAPDH Polyclonal Antibody, Unconjugated (bs-2188R) at 1:10000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.