bs-2190R [Primary Antibody]
pan-Cytokeratin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: pan-Cytokeratin

Immunogen Range: 101-200/403

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 16669

Swiss Prot: P19001

Source: KLH conjugated synthetic peptide derived from mouse Cytokeratin 19.

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background: Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Antibody to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 44

Cross Reactive Species: Human
Mouse
Horse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Xiaodong, et al. "The effect of porcine ADM to improve the burn wound healing." Int J Clin Exp Pathol 6.11 (2013): 2280-2291.Read more>>
  • Chen, Jia, et al. "Biological characterization of metanephric mesenchymal stem cells from the Beijing duck." Experimental and Therapeutic Medicine 11.2 (2016): 439-447.Read more>>
  • Sottnik et al. Osteocytes serve as a progenitor cell of osteosarcoma. (2014) J.Cell.Biochem. 115:1420-9Read more>>
  • Kammergruber E et al. Morphological and immunohistochemical characteristics of the equine corneal epithelium. Vet Ophthalmol. 2019 Feb 14.Read more>>
  • Yin X et al. Zinc oxide nanoparticles ameliorate collagen lattice contraction in human tenon fibroblasts. Arch Biochem Biophys. 2019 Jul 15;669:1-10. Read more>>
  • Chirackal RS et al. Urinary extracellular vesicles associated MCP-1 and NGAL derived from specific nephron segments differ between calcium oxalate stone formers and controls. Am J Physiol Renal Physiol. 2019 Aug 28. Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-pan-Cytokeratin Polyclonal Antibody, Unconjugated (bs-2190R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse stomach lysates probed with Anti-pan-Cytokeratin Polyclonal Antibody, Unconjugated (bs-2190R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.


A549 cell lysates probed with Anti-pan-Cytokeratin Polyclonal Antibody, Unconjugated (bs-2190R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.


MCF-7 cells probed with pan-Cytokeratin Polyclonal Antibody, FITC Conjugated (bs-2190R-FITC ) at 1:100 for 30 minutes compared to control cells (blue) and isotype control (orange).


Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human HepG2 cell lysates probed with pan-Cytokeratin Polyclonal Antibody, Unconjugated (bs-2190R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cytokeratin 19) polyclonal Antibody, Unconjugated (bs-2190R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with pan-Cytokeratin Polyclonal Antibody(bs-2190R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).