Host: Rabbit
Target Protein: EGFP
Specificity: This antibody will work best with EGFP that has a similar amino acid make-up as that of Aequorea Victoria.
Immunogen Range: 1-239/239
Clonality: Polyclonal
Isotype: IgG
Source: Purified EGFP synthetic protein homologous to EGFP naturally occurring in Aequorea victoria
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 27
Cross Reactive Species:
Human
Rat
Tags
(EGFP protein)
For research use only. Not intended for diagnostic or therapeutic use.
- Peng, Xu, et al. "Identification of ITGB4BP as a new interaction protein of P311." Life Sciences (2012).Read more>>
- Yoshida, Shuhei, et al. "Adipose-derived stem cell transplantation for therapeutic lymphangiogenesis in a mouse secondary lymphedema model."Regenerative medicine 10.5 (2015): 549-562.Read more>>
- Xie, Teng, et al. "Is X-linked methyl-CpG binding protein 2 a new target for the treatment of Parkinson's disease." Neural Regeneration Research 8.21 (2013): 1948.Read more>>
- Feng S et al. Development of a reverse genetics system for snakehead vesiculovirus (SHVV). (2018) Virology. Oct 15;526:32-37. Read more>>
- Feng S et al. Development of a reverse genetics system for snakehead vesiculovirus (SHVV). (2018) Virology. Oct 15;526:32-37. Read more>>
- Yang Sidi. et al. Salmonella Effector SpvB Inhibits NF-B Activity via KEAP1-Mediated Downregulation of IKK. Front Cell Infect Mi. 2021 Mar;11:207Read more>>
- Yongsen Wen. et al. Construction and immune evaluation of the recombinant duck adenovirus type 3 delivering capsid protein VP1 of the type 1 duck hepatitis virus. POULTRY SCIENCE. 2023 Sep;:103117Read more>>
Transfected HBX cells probed with Anti-EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
Formalin-fixed and paraffin embedded rat liver labeled with Anti-EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:300 followed by conjugation to the secondary antibody
Lane 1: GFP-Tagged fusion protein (full length) Overexpression E.coli lysates(Cat#: bs-33009P) probed with EGFP Polyclonal Antibody, Unconjugated (bs-2194R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.