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Host: Rabbit
Target Protein: phospho-STAT3 (Tyr705)
Modification Site: Tyr705
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6774
Swiss Prot: P40763
Source: KLH conjugated Synthesised phosphopeptide derived from human STAT3 around the phosphorylation site of Tyr705
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(1:200-1000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Predicted Molecular Weight: 85
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Sheep
Pig
Chicken
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Jisheng Wang. et al. Effects of Diabetes Mellitus on Sperm Quality in the Db/Db Mouse Model and the Role of the FoxO1 Pathway. Med Sci Monitor. 2021; 27: e928232-1Ce928232-12Read more>>
- Jiang-Tao Fan. et al. Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway. Neoplasia. 2021 Jul;23:692Read more>>
- Zhaxi Mima. et al. Blockade of JAK2 retards cartilage degeneration and IL-6-induced pain amplification in osteoarthritis. INT IMMUNOPHARMACOL. 2022 Dec;113:10934Read more>>
- Xiaoyan Song. et al. SRRM1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by regulating the JAK/STAT signaling pathway. TISSUE CELL. 2022 Oct;:101954Read more>>
- Dhanu Gupta. et al. Modulation of Pro-Inflammatory IL-6 Trans-Signaling Axis by Splice Switching Oligonucleotides as a Therapeutic Modality in Inflammation. CELLS-BASEL. 2023 Jan;12(18):2285Read more>>
- Xin Zeng. et al. -linolenic acid ameliorates pentylenetetrazol-induced neuron apoptosis and neurological impairment in mice with seizures via down-regulating JAK2/STAT3 pathway. BRIT J NUTR. 2024 May;:1-34Read more>>
- Zhong Zhuang. et al. Threonine Deficiency Increases Triglyceride Deposition in Primary Duck Hepatocytes by Reducing STAT3 Phosphorylation. INT J MOL SCI. 2024 Jan;25(15):8142Read more>>
- Ruoyun Dong. et al. Study on the mechanisms of defective spermatogenesis induced by TiO2 NPs based on 3D bloodtestis barrier microfluidic chip. TOXICOLOGY. 2024 Jul;:153888Read more>>
Lane 1: Human Jurkat cell lysates lysates; Lane 2: Jurkat treated with IFN-α(100 ng/ml，5 min) lysates probed with phospho-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-22386R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Placenta tissue lysates probed with phospho-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-22386R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (bs-22386R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT3 (Tyr705) alpha Polyclonal Antibody, Unconjugated (bs-22386R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT3 (Tyr705) alpha Polyclonal Antibody, Unconjugated (bs-22386R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT3 (Tyr705) alpha Polyclonal Antibody, Unconjugated (bs-22386R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT3 (Tyr705) alpha Polyclonal Antibody, Unconjugated (bs-22386R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-STAT3 (Tyr705) alpha Polyclonal Antibody, Unconjugated (bs-22386R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.