Host: Rabbit
Target Protein: phospho-STAT3 (Tyr705)
Modification Site: Tyr705
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6774
Swiss Prot: P40763
Source: KLH conjugated Synthesised phosphopeptide derived from human STAT3 around the phosphorylation site of Tyr705
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1mg/ml
Applications:
WB(WB=1:200-1000)
IHC-P(IHC-P=1:100-500)
Predicted Molecular Weight: 85
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Dog
Cow
Sheep
Pig
Chicken
Rabbit
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
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Lane 1: Human Jurkat cell lysates lysates; Lane 2: Jurkat treated with IFN-α(100 ng/ml，5 min) lysates probed with phospho-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-22386R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Placenta tissue lysates probed with phospho-STAT3 (Tyr705) Polyclonal Antibody, Unconjugated (bs-22386R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (bs-22386R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-STAT3 (Tyr705)) Polyclonal Antibody, Unconjugated (bs-22386R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.