bs-2240R [Primary Antibody]
MYL3/Myosin light chain 3 Antibody
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Host: Rabbit

Target Protein: MYL3/Myosin light chain 3

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4634

Source: KLH conjugated synthetic peptide derived from human MLC/Myosin light chain 3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. Human myosin light chain has clinical application as a cardiac marker.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat
Bovine
Dog
Pig
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-MYL3/Myosin light chain 3 Polyclonal Antibody, Unconjugated (bs-2240R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse heart labeled with Anti-MYL3/Myosin light chain 3 Polyclonal Antibody, Unconjugated (bs-2240R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Muscle lysates probed with MYL3/Myosin light chain 3 Polyclonal Antibody, Unconjugated (bs-2240R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Mouse Muscle tissue lysates; Lane 2: Rat Muscle tissue lysates probed with MYL3 Polyclonal Antibody, Unconjugated (bs-2240R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.