DATASHEET
Host:
Rabbit
Target Protein:
CD1a
Immunogen Range:
111-210/327
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
909
Swiss Prot:
P06126
Source:
KLH conjugated synthetic peptide derived from human CD1a
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background:
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
VALIDATION IMAGES
Lane 1: Human Molt-4 cell lysates; Lane 2: Human Jurkat cell lysates probed with CD1a Polyclonal Antibody,Unconjugated (bs-22573R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Jurkat cell lysates; Lane 2: Human Jurkat cell lysates probed with CD1a Polyclonal Antibody, Unconjugated (bs-22573R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with CD1a polyclonal antibody, unconjugated (bs-22573R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.