bs-22575R [Primary Antibody]
IKK gamma Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IKK gamma

Immunogen Range: 301-400/419


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8517

Swiss Prot: Q9Y6K9

Source: KLH conjugated synthetic peptide derived from human IKK gamma

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity (By similarity). Essential for viral activation of IRF3.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF()
ICC(1:100-500)

Predicted Molecular Weight: 46


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Cow
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IKK gamma Polyclonal Antibody, Unconjugated (bs-22575R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human pancreatic cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IKK gamma Polyclonal Antibody, Unconjugated (bs-22575R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: Human Hela cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human K562 cell lysates probed with IKK gamma Polyclonal Antibody, Unconjugated (bs-22575R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.