bs-2257R-Cy5 [Conjugated Primary Antibody]
SIRT1/sirtuin 1 Polyclonal Antibody, Cy5 Conjugated
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: SIRT1/sirtuin 1

Immunogen Range: 551-650/747


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23411

Swiss Prot: Q96EB6

Source: KLH conjugated synthetic peptide derived from human SIRT1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Conjugation: Cy5

Excitation/ Emission: 625,650nm/670nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 81


Cross Reactive Species: Human
Mouse
Dog

Predicted Cross Reactive Species: Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • "Liu GZ et al. Aldosterone stimulation mediates cardiac metabolism remodeling via Sirt1/AMPK signaling in canine model.Naunyn Schmiedebergs Arch Pharmacol. 2019 Mar 9."Read more>>
  • He Zhang. et al. Feeding frequency affects glucose and lipid metabolism through SIRT1/AMPK pathway in growing pigs with the same amount of daily feed. J Nutr Biochem. 2021 Nov;:108919Read more>>
  • Wan Boyang. et al. Zearalenone promotes follicle development through activating SIRT1/PGC-1_ signaling pathway in the ovaries of weaned gilts. J Anim Sci. 2022 FebRead more>>
  • Cuiting Liao. et al. Inhibition of NAD kinase elevates the hepatic NAD+ pool and alleviates acetaminophen-induced acute liver injury in mice. BIOCHEM BIOPH RES CO. 2022 Jul;612:7Read more>>
  • Yu Wang. et al. Seabuckthorn Reverses High-Fat-Diet-Induced Obesity and Enhances Fat Browning via Activation of AMPK/SIRT1 Pathway. NUTRIENTS. 2022 Jan;14(14):2903Read more>>
  • Wang, Daquan. et al. Protection of zero-valent iron nanoparticles against sepsis and septic heart failure. J NANOBIOTECHNOL. 2022 Dec;20(1):1-22Read more>>
  • Hongye Liu. et al. The ginsenoside Rh2 protects porcine oocytes against aging and oxidative stress by regulating SIRT1 expression and mitochondrial activity. THERIOGENOLOGY. 2023 Apr;200:125Read more>>
  • Kaihui Xie. et al. Overexpression of SIRT1 alleviates oxidative damage and barrier dysfunction in CPB2 toxin-infected IPEC-J2 cells. MICROB PATHOGENESIS. 2023 Jun;:106181Read more>>
  • Lijuan Bao. et al. Hexadecanamide alleviates Staphylococcus aureus-induced mastitis in mice by inhibiting inflammatory responses and restoring blood-milk barrier integrity. PLOS PATHOG. 2023 Nov;19(11):e1011764Read more>>
  • Wangrui Lei. et al. Isopropyl 3-(3,4-dihydroxyphenyl) 2-hydroxypropanoate protects septic myocardial injury via regulating GAS6/Axl-AMPK signaling pathway. BIOCHEM PHARMACOL. 2024 Feb;:116035Read more>>
VALIDATION IMAGES