bs-22741R [Primary Antibody]
PAX7 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PAX7

Immunogen Range: 141-240/520


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5081

Swiss Prot: P23759

Source: KLH conjugated synthetic peptide derived from human PAX7

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Probable transcription factor. May have a role in myogenesis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)

Predicted Molecular Weight: 55


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Cow
Sheep
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Weng S et al. Improvement of muscular atrophy by AAVCSaCas9-mediated myostatin gene editing in aged mice. Cancer Gene Ther. 2020 Dec;27(12):960-975.Read more>>
  • Hanhao Dai. et al. Inhibiting uptake of extracellular vesicles derived from senescent bone marrow mesenchymal stem cells by muscle satellite cells attenuates sarcopenia. J ORTHOP TRANSL. 2022 Jul;35:23Read more>>
  • Hanhao Dai. et al. Hierarchically injectable hydrogel sequentially delivers antagomiR-467a-3p-loaded and antagomiR-874-5p-loaded satellite-cell-targeting bioengineered extracellular vesicles attenuating sarcopenia. ADV HEALTHC MATER. 2023 Feb;:2203056Read more>>
  • Siqi Liu. et al. Estrogen-mediated oar-miR-485-5p targets PPP1R13B to regulate myoblast proliferation in sheep. INT J BIOL MACROMOL. 2023 May;236:123987Read more>>
  • Qian Chen. et al. Transcriptomic Analysis Reveals mRNA and Alternative Splicing Events in Ovine Skeletal Muscle Satellite Cells during Proliferation and Differentiation. ANIMALS. 2023 Jan;13(6):1076Read more>>
  • Xu Enbo. et al. Tissue-like cultured fish fillets through a synthetic food pipeline. npj Science of Food. 2023 May;7(1):1-13Read more>>
  • Shengliang Zhang. et al. Characterization of Proliferation Medium and Its Effect on Differentiation of Muscle Satellite Cells from Larimichthys crocea in Cultured Fish Meat Production. Fishes. 2023 Sep;8(9):429Read more>>
  • Ke Shenghui. et al. Isolation, identification, and induced differentiation of satellite cells from skeletal muscle of adult tree shrews. IN VITRO CELL DEV-AN. 2023 Dec;:1-18Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse embryo; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PAX7 Polyclonal Antibody, Unconjugated (bs-22741R) at 1:2000 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PAX7 Polyclonal Antibody, Unconjugated (bs-22741R) at 1:2000 overnight at 4°C, DAB staining.