Host: Rabbit
Target Protein: RSL1D1
Immunogen Range: 311-410/452
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 66409
Swiss Prot: Q8BVY0
Source: KLH conjugated synthetic peptide derived from mouse RSL1D1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
IHC-P(1:200-400)
Predicted Molecular Weight: 55
Cross Reactive Species:
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with RSL1D1 Polyclonal Antibody, Unconjugated (bs-22952R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Rat Heart lysates; Lane 2: Mouse Heart lysates; Lane 3: Rat Muscle lysates; Lane 4: Mouse Muscle lysates; Lane 5: Rate Placenta lysates; Lane 6: Mouse Placenta lysates; Lane 7: Rat Cerebrum lysates; Lane 8: Mouse Cerebrum lysates; Lane 9: Rat Testis lysates; Lane 10: Mouse Testis lysates; Lane 11: Rat Uterus lysates; Lane 12: Mouse Uterus lysates probed with RSL1D1 Polyclonal Antibody, Unconjugated (bs-22952R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.