DATASHEET
Host:
Rabbit
Target Protein:
Ku70 / XRCC6
Immunogen Range:
231-330/609
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
2547
Swiss Prot:
P12956
Source:
KLH conjugated synthetic peptide derived from human Ku-70
Purification:
Purified by Protein A.
Storage Buffer:
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Background:
Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
Conjugation:
FITC
Excitation/ Emission:
494nm/518nm
Size:
100µL
Concentration:
1ug/ul
Applications:
WB(1:300-5000)
IF(ICC)(1:50-200)
Human
Predicted Cross Reactive Species:
Mouse
Rat
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.