bs-23011R [Primary Antibody]
GDF8 Polyclonal Antibody
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Host: Rabbit

Target Protein: GDF8

Immunogen Range: 281-350/375


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2660

Swiss Prot: O14793

Source: KLH conjugated synthetic peptide derived from human GDF8

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth. Acts specifically as a negative regulator of skeletal muscle growth. [SUBUNIT] Homodimer; [TISSUE SPECIFICITY] Expressed specifically in developing and adult skeletal muscle. Weak expression in adipose tissue. Belongs to the TGF-beta family.

Size: 100µL

Concentration: 1ug/ul

Applications: IHC-P(1:200-400)
IHC-F(1:100-500)
IF(ICC)(1:50-200)
IF(1:50-200)

Predicted Molecular Weight: 12/43 kDa


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDF8) ) Polyclonal Antibody, Unconjugated (bs-23011R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GDF8) ) Polyclonal Antibody, Unconjugated (bs-23011R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.