bs-23012R [Primary Antibody]
GDF8 Polyclonal Antibody
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Host: Rabbit

Target Protein: GDF8

Immunogen Range: 301-375/375


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2660

Swiss Prot: O14793

Source: KLH conjugated synthetic peptide derived from human GDF8

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. This gene is thought to encode a secreted protein which negatively regulates skeletal muscle growth. Acts specifically as a negative regulator of skeletal muscle growth. [SUBUNIT] Homodimer; [TISSUE SPECIFICITY] Expressed specifically in developing and adult skeletal muscle. Weak expression in adipose tissue. Belongs to the TGF-beta family.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 43


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Sheep
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Weng S et al. Improvement of muscular atrophy by AAVCSaCas9-mediated myostatin gene editing in aged mice. Cancer Gene Ther. 2020 Dec;27(12):960-975.Read more>>
  • Weng, Shaoting. et al. Construction of a rAAV-SaCas9 system expressing eGFP and its application to improve muscle mass. 2021 Sep 29Read more>>
  • Zhao Jiamin. et al. Sea buckthorn oil regulates primary myoblasts proliferation and differentiation in vitro. IN VITRO CELL DEV-AN. 2023 Dec;:1-12Read more>>
VALIDATION IMAGES

Lane 1: Mouse Muscle lysates; Lane 2: Mouse Embryo lysates; Lane 3: Mouse Tongue lysates; Lane 4: Rat Muscle lysates; Lane 5: Rat Tongue lysates; Lane 6: Rat Uterus lysates probed with GDF8 Polyclonal Antibody, Unconjugated (bs-23012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-GDF8) ) Polyclonal Antibody, Unconjugated (bs-23012R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-GDF8) ) Polyclonal Antibody, Unconjugated (bs-23012R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Anti-GDF8) ) Polyclonal Antibody, Unconjugated (bs-23012R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.