bs-23016R [Primary Antibody]
Cyclin B1 Polyclonal Antibody
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Host: Rabbit

Target Protein: Cyclin B1

Immunogen Range: 351-433/433


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 891

Swiss Prot: P14635

Source: KLH conjugated synthetic peptide derived from human Cyclin B1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].

Size: 100uL, 20uL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(ICC)(1:50-200)
IF(1:50-200)

Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Cow
Sheep
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cuicui Zhuang. et al. Escherichia coli infection mediates pyroptosis via activating p53-p21 pathway-regulated apoptosis and cell cycle arrest in bovine mammary epithelial cells. MICROB PATHOGENESIS. 2023 Sep;:106338Read more>>
  • Gong Zhian. et al. IFRD2, a target of miR-2400, regulates myogenic differentiation of bovine skeletal muscle satellite cells via decreased phosphorylation of ERK1/2 proteins. J MUSCLE RES CELL M. 2024 Jun;:1-1Read more>>
VALIDATION IMAGES

Lane 1: Mouse NIH/3T3 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human U-2OS cell lysates; Lane 5: Human K562 cell lysates probed with Cyclin B1 Polyclonal Antibody, Unconjugated (bs-23016R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.