Host: Rabbit
Target Protein: Vimentin
Immunogen Range: 101-200/466
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7431
Swiss Prot: P08670
Source: KLH conjugated synthetic peptide derived from human Vimentin
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 53
Cross Reactive Species: Human
Predicted Cross Reactive Species:
Mouse
Rat
Cow
Sheep
Horse
For research use only. Not intended for diagnostic or therapeutic use.
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Lane 1: U2OS Cell lysates; Lane 2: U87MG Cell lysates; Lane 3: HeLa lysates; Lane 4: Jurkat lysates; probed with Vimentin Polyclonal Antibody, unconjugated (bs-23063R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded human cervical cancer section; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Vimentin Polyclonal Antibody, Unconjugated (bs-23063R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Hela Cell lysate; Probed with Vimentin Polyclonal Antibody, Unconjugated (bs-23063R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.