bs-23102R
[Primary Antibody]
Ki-67 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Ki-67
Immunogen Range: 2901-3000/3256
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4288
Swiss Prot: P46013
Source: KLH conjugated synthetic peptide derived from human Ki-67
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 358
Cross Reactive Species:
Human
Mouse
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Fu R et al. Avenanthramide A induces cellular senescence via miR-129-3p/Pirh2/p53 signaling pathway to suppress colon cancer growth. Agric Food Chem. 2019 May 1;67(17):4808-4816.Read more>>
- Jian-Lin Zhou. et al. CircSPI1_005 ameliorates osteoarthritis by sponging miR-370-3p to regulate the expression of MAP3K9. INT IMMUNOPHARMACOL. 2022 Sep;110:109064Read more>>
- Yanwen Liu. et al. MicroRNA-200c-5p Regulates Migration and Differentiation of Myoblasts via Targeting Adamts5 in Skeletal Muscle Regeneration and Myogenesis. INT J MOL SCI. 2023 Jan;24(5):4995Read more>>
- Danyang Fan. et al. Regulation of myo-miR-24-3p on the Myogenesis and Fiber Type Transformation of Skeletal Muscle. GENES-BASEL. 2024 Mar;15(3):269Read more>>
VALIDATION IMAGES
Mouse lymph node lysates probed with Ki-67 Polyclonal Antibody, Unconjugated (bs-23102R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Ki-67) polyclonal Antibody, Unconjugated (bs-23102R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-AF488 antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Ki-67) polyclonal Antibody, Unconjugated (bs-23102R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-AF488 antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Ki-67) polyclonal Antibody, Unconjugated (bs-23102R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-AF488 antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Ki-67) polyclonal Antibody, Unconjugated (bs-23102R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-AF488 antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Ki-67) polyclonal Antibody, Unconjugated (bs-23102R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-AF488 antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Ki-67) polyclonal Antibody, Unconjugated (bs-23102R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-AF488 antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Ki-67 Polyclonal Antibody(bs-23102R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).