bs-23102R-A750 [Conjugated Primary Antibody]
Ki-67 Polyclonal Antibody, ALEXA FLUOR® 750 Conjugated
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: Ki-67

Immunogen Range: 2901-3000/3256


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4288

Swiss Prot: P46013

Source: KLH conjugated synthetic peptide derived from human Ki-67

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Conjugation: ALEXA FLUOR® 750

Excitation/ Emission: 749nm/775nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 358


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Fu R et al. Avenanthramide A induces cellular senescence via miR-129-3p/Pirh2/p53 signaling pathway to suppress colon cancer growth. Agric Food Chem. 2019 May 1;67(17):4808-4816.Read more>>
  • Jian-Lin Zhou. et al. CircSPI1_005 ameliorates osteoarthritis by sponging miR-370-3p to regulate the expression of MAP3K9. INT IMMUNOPHARMACOL. 2022 Sep;110:109064Read more>>
  • Yanwen Liu. et al. MicroRNA-200c-5p Regulates Migration and Differentiation of Myoblasts via Targeting Adamts5 in Skeletal Muscle Regeneration and Myogenesis. INT J MOL SCI. 2023 Jan;24(5):4995Read more>>
  • Danyang Fan. et al. Regulation of myo-miR-24-3p on the Myogenesis and Fiber Type Transformation of Skeletal Muscle. GENES-BASEL. 2024 Mar;15(3):269Read more>>
VALIDATION IMAGES