Host: Rabbit
Target Protein: FOXO1
Immunogen Range: 231-330/655
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2308
Swiss Prot: Q12778
Source: KLH conjugated synthetic peptide derived from human FOXO1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 70
Cross Reactive Species:
Human
Mouse
Predicted Cross Reactive Species:
Rat
Cow
Sheep
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Ming-Feng He. et al. Coptisine Inhibits Influenza Virus Replication by Upregulating p21. MOLECULES. 2023 Jan;28(14):5398Read more>>
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FOXO1 Polyclonal Antibody(bs-23175R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).