bs-2329R [Primary Antibody]
PKC epsilon Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PKC epsilon

Immunogen Range: 631-737/737


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5581

Swiss Prot: Q02156

Source: KLH conjugated synthetic peptide derived from human PKCE (AGC-kinase C-terminal)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 81


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Qiao et al. Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. (2016) Oxid.Med.Cell.Longe. 2016:5139458Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-PKC epsilon Polyclonal Antibody, Unconjugated (bs-2329R) followed by conjugation to the secondary antibody


Formalin-fixed and paraffin embedded rat Testis tissue labeled with Anti-PKC epsilon Polyclonal Antibody, Unconjugated(bs-2329R) 1:300 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-PKC epsilon Polyclonal Antibody, Unconjugated(bs-2329R) 1:300 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded Human kidney tissue labeled with Anti-PKC epsilon Polyclonal Antibody, Unconjugated(bs-2329R) 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates probed with PKC epsilon Polyclonal Antibody, Unconjugated (bs-2329R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.