bs-23329R [Primary Antibody]
SMAD6 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: SMAD6

Immunogen Range: 351-450/469


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4091

Swiss Prot: O43541

Source: KLH conjugated synthetic peptide derived from human SMAD6

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014]

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 54 kDa


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jianbo Feng. et al. LRRC4 mediates the formation of circular RNA CD44 to inhibit GBM cell proliferation. Mol Ther-Nucl Acids. 2021 AugRead more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin-embedded mouse uterus tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SMAD6 Polyclonal Antibody, Unconjugated (bs-23329R) at 1:400 overnight at 4°C, followed by secondary antibody and DAB staining.