bs-2332R [Primary Antibody]
Diglyceride acyltransferase Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DGAT1/Diglyceride acyltransferase

Immunogen Range: 251-350/488


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8694

Swiss Prot: O75907

Source: KLH conjugated synthetic peptide derived from human DGAT1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 55


Cross Reactive Species: Human
Mouse
Rat
Dog

Predicted Cross Reactive Species: Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Li et al. Increase of Autonomic Nerve Factors in Epicardial Ganglionated Plexi During Rapid Atrial Pacing Induced Acute Atrial Fibrillation. (2017) Med.Sci.Monit. 23:3657-3665Read more>>
  • Yankun Wang. et al. Investigation of seasonal changes in lipid synthesis and metabolism-related genes in the oviduct of Chinese brown frog (Rana dybowskii). EUR J HISTOCHEM. 2023 Oct 2; 67(4): 389Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti DGAT1/Diglyceride acyltransferase Polyclonal Antibody, Unconjugated (bs-2332R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti DGAT1/Diglyceride acyltransferase Polyclonal Antibody, Unconjugated (bs-2332R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DGAT1 Polyclonal Antibody, Unconjugated (bs-2332R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.


Lane 1: Mouse Large intestine lysates; Lane 2: Mouse intestine lysates probed with DGAT1 Polyclonal Antibody, Unconjugated (bs-2332R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Adipose tissue lysates; Lane 2: Mouse Adrenal gland tissue lysates; Lane 3: Rat Adipose tissue lysates; Lane 4: Rat Stomach tissue lysates ; Lane 5: Rat Testis tissue lysates ; Lane 6: Human HL60 cell lysates; Lane 7: Human HepG2 cell lysates probed with DGAT1 Polyclonal Antibody, Unconjugated (bs-2332R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.