bs-23335R [Primary Antibody]
DNase II Polyclonal Antibody
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: DNase II

Immunogen Range: 201-300/360


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1777

Swiss Prot: O00115

Source: KLH conjugated synthetic peptide derived from human DNase II

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)

Predicted Molecular Weight: 38


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse stomach lysates; Lane 3: Mouse Liver lysates; Lane 4: Mouse Kidney lysates ; Lane 5: Mouse Testis lysates;Lane 6: Rat Cerebrum lysates; Lane 7: Rat Liver cell lysates; Lane 8: Rat Kidney lysates; Lane 9: Human A431 cell lysates; Lane 10: Human Huvec lysates probed with DNase II Polyclonal Antibody, Unconjugated (bs-23335R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.