bs-23376R [Primary Antibody]
Progesterone Receptor Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Progesterone Receptor

Immunogen Range: 221-320/933


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5241

Swiss Prot: P06401

Source: KLH conjugated synthetic peptide derived from human Progesterone Receptor

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Estrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboxy terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER alpha and ER beta. ER alpha and ER beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-Aand hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 103


Cross Reactive Species: Human
Mouse
Rat
Sheep

Predicted Cross Reactive Species: Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Duan H et al. Expression of estrogen receptor, androgen receptor, and progesterone nuclear receptor in sheep uterus during the estrous cycle. Reprod Domest Anim. 2019 Jun 12. Read more>>
  • Junjie Hu. et al. Assessment of progesterone synthesis and its regulation role on dihydrotestosterone secretion in sheep epididymis. Gene. 2021 Jul;790:145699Read more>>
  • Ding Ziqiang. et al. Regulation of progesterone during follicular development by FSH and LH in sheep. ANIM REPROD. 2022 Jul;19Read more>>
  • Hyun Jin Kim. et al. Dienogest May Reduce Estradiol- and Inflammatory Cytokine-Induced Cell Viability and Proliferation and Inhibit the Pathogenesis of Endometriosis: A Cell Culture- and Mouse Model-Based Study. BIOMEDICINES. 2022 Nov;10(11):2992Read more>>
  • Hongwei Duan. et al. The effect of melatonin on sheep endometrial epithelial cell apoptosis through the receptor and non-receptor pathways. GEN COMP ENDOCR. 2022 Nov;:114182Read more>>
  • Jianlin Zeng. et al. Subacute Ruminal Acidosis as a Potential Factor That Induces Endometrium Injury in Sheep. INT J MOL SCI. 2023 Jan;24(2):1192Read more>>
  • Yu Tang. et al. hUMSCs Restore Uterine Function by Inhibiting Endometrial Fibrosis via Regulation of the MMP-9/TIMP-1 Ratio in CDDP-Induced Injury Rats. STEM CELLS INT. 2023;2023:8014052Read more>>
  • Polat Blent. et al. The effects of low-level laser therapy on polycystic ovarian syndrome in rats: three different dosages. LASER MED SCI. 2023 Dec;38(1):1-7Read more>>
  • Zilu Wang. et al. Establishment of an immortalized cell line derived from human adenomyosis ectopic lesions. TISSUE CELL. 2023 Dec;:102284Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:400 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:400 overnight at 4°C, DAB staining.


Mouse cerebllumlysates probed with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Progesterone Receptor Polyclonal Antibody, Unconjugated (bs-23376R) at 1:200 overnight at 4°C, DAB staining.