bs-23398R
[Primary Antibody]
PPAR alpha Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: PPAR alpha
Immunogen Range: 181-280/468
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 5465
Swiss Prot: Q07869
Source: KLH conjugated synthetic peptide derived from human PPAR alpha
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Peroxisome proliferators are nongenotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 51
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Sheep
Pig
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Dong S et al. Dihydromyricetin alleviates acetaminophen-induced liver injury via the regulation of transformation, lipid homeostasis, cell death and regeneration. Life Sci. 2019 Jun 15;227:20-29. Read more>>
- Jin X et al. Fine particles cause the abnormality of cardiac ATP levels via PPARɑ-mediated utilization of fattyacid and glucose using in vivo and in vitro models.Environ Pollut. 2019 Jun;249:286-294. Read more>>
- Bian X et al. Periostin contributes to renal and cardiac dysfunction in rats with chronic kidney disease: reduction of PPARα.Biochimie. 2019 May;160:172-182.Read more>>
- Pan Y et al. Regulatory effect of Grifola frondosa extract rich in polysaccharides and organic acids on glycolipid metabolism and gut microbiota in rats. Int J Biol Macromol. 2019 Nov 8. pii: S0141-8130(19)37563-4. Read more>>
- L.X. Li. et al. MiR-23a-5p exacerbates intestinal ischemiaCreperfusion injury by promoting oxidative stress via targeting PPAR alpha. Biochem Pharmacol. 2020 Oct;180:114194Read more>>
- Huiying Gu. et al. Soluble Klotho Improves Hepatic Glucose and Lipid Homeostasis in Type 2 Diabetes. Mol Ther-Meth Clin D. 2020 Sep;18:811Read more>>
- Zang Zhen-Zhong. et al. Uncovering the Protective Mechanism of the Volatile Oil of Acorus tatarinowii against Acute Myocardial Ischemia Injury Using Network Pharmacology and Experimental Validation. Evid-Based Compl Alt. 2021;2021:6630795Read more>>
- Dongmin Zou. et al. BHBA regulates the expressions of lipid synthesis and oxidation genes in sheep hepatocytes through the AMPK pathway. Res Vet Sci. 2021 Nov;140:153Read more>>
- Xiaohui Xu. et al. Hexafluoropropylene oxide dimer acid (HFPO-DA) induced developmental cardiotoxicity and hepatotoxicity in hatchling chickens: Roles of peroxisome proliferator activated receptor alpha. Environ Pollut. 2021 Dec;290:118112Read more>>
- Pan Cao. et al. Pinocembrin ameliorates acute liver failure via activating the Sirt1/PPAR pathway in vitro and in vivo. Eur J Pharmacol. 2022 Jan;915:17461Read more>>
- Yue-Qiang Huang. et al. Di-2-ethylhexyl phthalate (DEHP) induced lipid metabolism disorder in liver via activating the LXR/SREBP-1c/PPAR_/_ and NF-_B signaling pathway. FOOD CHEM TOXICOL. 2022 Jul;165:113119Read more>>
- Pan Cao. et al. Sirtuin1 attenuates acute liver failure by reducing reactive oxygen species via hypoxia inducible factor 1_. WORLD J GASTROENTERO. 2022 May 7; 28(17): 17981813Read more>>
- Hao Ni. et al. Long term toxicities following developmental exposure to perfluorooctanoic acid: Roles of peroxisome proliferation activated receptor alpha. ENVIRON POLLUT. 2023 Jan;317:120722Read more>>
- Xiaoyu Qu. et al. Integration of metabolomics and proteomics analysis to explore the mechanism of neurotoxicity induced by receipt of isoniazid and rifampicin in mice. NEUROTOXICOLOGY. 2023 Jan;94:24Read more>>
- Qixuan Dong. et al. Hexafluoropropylene oxide tetramer acid (HFPO-TeA)-induced developmental toxicities in chicken embryo: Peroxisome proliferator-activated receptor Alpha (PPAR) is involved. ECOTOX ENVIRON SAFE. 2023 Mar;253:114671Read more>>
- Yongzhi Sun. et al. GB1a Activates SIRT6 to Regulate Lipid Metabolism in Mouse Primary Hepatocytes. INT J MOL SCI. 2023 Jan;24(11):954Read more>>
- Lijuan Bao. et al. Hexadecanamide alleviates Staphylococcus aureus-induced mastitis in mice by inhibiting inflammatory responses and restoring blood-milk barrier integrity. PLOS PATHOG. 2023 Nov;19(11):e1011764Read more>>
- W.J. Tu. et al. Osteocalcin activates lipophagy via the ADPN-AMPK/PPAR-mTOR signaling pathway in chicken embryonic hepatocyte. POULTRY SCIENCE. 2023 Nov;:103293Read more>>
VALIDATION IMAGES
Mouse heart lysates probed with PPAR alpha Polyclonal Antibody, Unconjugated (bs-23398R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.